ELISAAPSubstrate
Lumi-Phos530isaready-to-usechemiluminescentreagentformulationcontainingthealkalinephosphatase(AP)substrate,lumigenPPD.Lumi-Phos530isusefulforchemiluminescentdetectionofAPconjugatesinimmunoassaysandDNAprobeassays.Itprovidesultrasensitivedetectionofanalytescapturedonmagneticparticlesandinmicrotiterplatewells.Lumi-Phos530is10,000timesmoresensitivethancolorimetricsubstratesinsolution.
- Excellentsensitivity-lowpicogramtofemtogram
- Sustainedluminescence-withlowconcentrationsofAP,thelightintensityremainsconstantforseveralhours
- Linearcalibrationcurves-theluminescenceintensityatanytimepointisadirectmeasureoftheconcentrationoftheenzyme
- HighstABIlityandlongshelflife
ChemiluminescentEnhancement
Auniqueco-surfactantsystempromotesandenhancesenergy-transfertothefluorescentheadgroupscausingthegenerationofluminescencewithabroademissionmaximumat530nm.