HomogeneousImmunoassay
lumigenSPARCLisahomogeneousproximityassaytechnologyutilizingflashchemiluminescencedetectionwithoutsolidsupportorwashstepsallowingforassaystobecompletedinlessthan30minutes.TheSPARCLtechnologyenablesassaystobeminiaturizedforhighthroughputscreeningwhilemaintainingsensitiveresultswithgooddynamicrange.SPARCLcanbeautomatedandadaptedformultipleapplicationsincludingELISA,protein-proteinandprotein-nucleicacidinteractions,andhighthroughputbindingassays.
- Completeassaysinlessthan30minutes-Savetimewithonlyoneincubationstep,noseparationorwashstepsandinstantaneoussignalgenerationfromflashchemiluminescence
- Costeffective-Usefewerreagentsandinstrumentswithnoneedforplate/beadcoatingoperations,automatedwashingstationsorotherexpensivedetectionequipment.
- FlexIBLeassayformats-Adaptableformanydifferentkindsofassaysinsolutionorsolidphaseformatstostudyawidevarietyoftargets
- Producelesswaste-Nowashmeansnodisposalofwashsolution.SPARCLproducesonly1xwellvolumeofwastecomparedto13xwellvolumeforconventionalELISAassays.
SPARCLFormats
Solutionorsolidphaseassay
SPARCLcanbeimplementedinformatswithorwithoutasolidphase.Whenusingasolidphase,boththeacridancompoundandaspecificcaptureantibodyarecoupledtosolidphasessuchasmicroparticlesormicrotiterplates,whereaswhenthesolidphaseisomittedthecaptureantibodyisdirectlylabeledwiththeacridancompound.Thesolutionphaseformateliminatestheneedforplateorparticlecoating,improveskineticstoshortenincubationtimeandduetothelackofasolid/solutioninterfaceoffersamorenativeBIOLOGicalenvironment.
SPARCLTechnology
SPARCL(SpatialProximityAnalyteReagentCaptureLuminescence)isaproximitydependentchemiluminescenttechnologyforthedetectionofspecificbindinginteractionorassociationbetweentwobindingpartners.InaSPARCLassay,abindingpartnerlabeledwithachemiluminescentsubstrate(acridan)andasecondbindingpartnerlabeledwithhorserADIshperoxidase(HRP)arebroughtintocloseproximitytoeachotherthroughaspecificbindingevent.BecauseofthiscloseproximityofacridantotheHRPenzymeaflashofchemiluminescenceisgenerateduponadditionofatriggersolutioncontaininghydrogenperoxideandanenhancer.
