WesternBlottingHRPSubstrate
lumigenECLExtra(TMA-3)isaproprietaryacridanbasedchemiluminescentsubstrateforhighlysensitivedetectionofWesternblottedandELISAcapturedproteinsthatareboundtohorserADIshperoxidase(HRP)labeledantibodies.
- Veryrapidonsetofchemiluminescence-imagescanberecordedinseconds
- Excellentsensitivity-lowpicogramtomidfemtogram
- Reducedantibodyusage-morethan10-foldreductionintheusageofexpensiveantibodyconjugates
- Temperatureinsensitive-analyticalresultsareinsensitivetotemperaturesfrom22°C-35°Creducingtheneedforprecisetemperaturecontrol
- Sustainedsignalduration -overseveralhoursallowsconvenientimaging
StableWorkingSolution
AsignificantfeatureofLumigenECLExtraisitsexcellentstABIlity.Unlikemostotherchemiluminescentperoxidasesubstrates,theworkingsolutionisstableatroomtemperatureforatleastoneweekandat2-8°Cforatleastonemonth.Withthisstability,theworkingsolutioncanbepreparedandstoredforlateruse.
RapidPeakIntensity
ReactionofthesubstratewithHRPenzymegeneratesrapidluminescenceofveryhighintensitywithinseconds.Peakintensityoflightemissionoccursat440nm.