LumigenHyPerBlu is a novel ready-to-use substrate for the direct chemiluminescent detection of hydrogen peroxide. Reaction of the substrate with hydrogen peroxide rapidly generates sustained high-intensity luminescence for maximum detection sensitivity in solution assays. Coupled with oxidases, HyPerBlu allows one step indirect quantitation of oxidase substrates or the oxidase itself.
umigen PPD Lumigen® PPD (4-methoxy-4-(3-phosphatephenyl)spiro [1,2-dioxetane-3,2'-adamantane], disodium salt) is used for ultrasensitive chemiluminescent detection of alkaline phosphatase in immunoassays and DNA probe assays using magnetic particles, beads, tubes and microtiter plates as well as nylon and PVDF membranes.