OTHERTOOLSANDRESOURCES  

 

 

 

CHEMICALSTRUCTURE

 

 

 

 3,5-difluoro-4-hydroxybenzylideneimidazolinone-2-oxime(DHFO)

 

 

MOLECULARFORMULA:

C₁₂H₈F₂N₃O₃

 

MOLECULARWEIGHT:

281.2

 

CASNAME/NUMBER:

(E)-4-((Z)-3,5-difluoro-4-hydroxybenzylidene)-1-methyl-5-oxo-4,5-dihydro-1H-imidazole-2-carbaldehydeoxime / 

 

 

 

 

  

FLUORESCENCESPECTRA

 

A.AbsorptionandfluorescenceemissionspectraofCorn^TM/DFHOinpH7.4buffer.  B. AbsorptionandfluorescenceemissionspectraofBroccoli^TM/DFHOinpH7.4buffer.  

 

 

PRESENTATION: Lyophilizeddye

  

 

 

QUALITYASSURANCE: Productsareanalyzedby ¹HNMRandLC-MSandprovidedatpurityof>95%byHPLC. 

 

 

USAGESTATEMENT: Thisproductisintendedforresearchuseonlyandarenottobeusedforanyotherpurpose,whichincludesbutisnotlimitedto,unauthorizedcommercialuses,invitrodiagnosticuses,exvivoorinvivotherapeuticusesoranytypeofconsumptionorapplicationtohumansoranimals.Duetothehighlyspecificnatureoffluorophoresandaptamers,wecannotpredictorbeheldresponsIBLewithrespecttohowLucerna^TM productswillbehaveinitscustomers"systems.ResearchersusingLucerna^TM productsshouldconductoptimizationstudiestoachievetheoptimalresultpossiblefortheirintendedapplication. 

 

 

  

 

DFHOIMAGES 

 

 

A. Live-cellimagingofHEK293TcellsexpressingU6promoterdrivenCorn^TM inthepresenceof10µMDFHO.ImagesareHoechst-stainednuclei(blue)andCorn^TM fluorescence(yellow),individuallyoroverlayed. ImageswereacquiredusingYFPandDAPIfiltersets.  B. Live-cellimagingofE.colicellsexpressingtRNApromoterdrivenCorn^TM inthepresenceof10µMDFHO.

  

 

REFERENCEFORTHEPRODUCT:

 

SongW,FilonovGS,KimH,HirschM,LiX,MoonJD,JaffreySR.2017. ImagingRNApolymeraseIIItranscriptionusingaphotostableRNA-fluorophorecomplex. NatChemBiol.

 

 

 

THISPRODUCTWASCITEDIN: 

 

 

 

LICENSING: 

 

Lucernahastheexclusiverightstoallpatentscoveringtheuseofthisproduct. AllcommercialusersshouldcontactLucernaforauselicense. 

  

 

 

Lucernal Lu`cer´nal a. 1. Of or pertaining to a lamp. Lucernal microscope a form of the microscope in which the object is illuminated by means of a lamp, and its image is thrown upon a plate of ground glass connected with the instrument, or on a screen independent of it. Webster's Revised Unabridged Dictionary, published 1913 by G. & C. Merriam Co. Want to thank TFD for its existence? Tell a friend about us, add a link to this page, or visit the webmaster's page for free fun content. Link to this page: