pH:Varies
Conductance:Varies
Appearance:ClearSolutions
Features
- 384Crystallizationconditions,optimized throughhighthroughputvalidation
- Derivedfromtrialsofmorethan40,000diverseproteinsat theMidwestCenterforStructuralGenomics
- Broadcoverageofcrystallizationspace
- Derivedfromcrystallizationoffrombothprokaryoticandeukaryoticorganisms
TheMCSGSuitehasbeendevelopedattheMidwestCenterforStructuralGenomics.AspartoftheProteinStructureInitiative,MCSGhasbeeninstrumentalinthedevelopmentofrapid,integratedandcost-effectivemethodsforproteinstructuredeterminationviaX-raycrystallography.
Throughextensivecrystallizationscreeningofmorethan40,000diverseproteinsfrombothprokaryoticandeukaryoticorganisms,researchersattheMCSGhavederivedthemostup-to-datesetofcrystallizationconditionsforinitialscreening.Thesenon-redundantconditionsconstitutethebroadestcoverageofcrystallizationspaceofanycommerciallyavailablescreenandaresupportedbyadecadeofexperimentalanalysisandvalidation.
ThecompleteMCSGsuite(384conditions)comprisesof4screensof96uniqueconditionsthatareavailableeitherasdeep-wellblocks(1.7mLperwell)ortubes(10ml). Conditions1-288areorganizedinorderofdecreasingcrystalproductivityinscreensMCSG-1,MCSG-2andMCSG-3. ThusMCSG-1:A1conditionhasyieldedthegreatestnumberofuniquecrystalsforalltargetsscreenedwiththesuitepredecessors. TheconditionsthatcompriseMCSG-4havebeenrationallyselectedtosystematicallysampleregionsofcrystallizationspaceforproteinsrequiringadditionalscreening.
Theendresultisthebest,first-passscreeningsuitepossIBLe,with:
- Data-drivenselectionindividualconditionsensuringconditionswithhighproductivitypotentialwerenotaccidentallyexcluded
- Superiororganization
- ThebroadestcoverageofcrystallizationspacewithinareasonablenumberofstartingconditionsformaximalefficiencywhilemaintainingapplicABIlityformoreuniqueanddifficulttargets.
