WiththesoftwaremoduleLeicaApplicationSuiteX(LASX)3DAnalysisyoucanmeasurevariousaspectsofintracellularstructuressuchasvolumesofnuclei,theirsurface,ordistancesandanglesbetweenthenuclei.Individual3Dobjectscanbemeasuredwiththeinteractive3Dmeasurementtool.Theanalysiswizardguidesyouthroughthe3Danalysisstepbysteptoobtainautomatedmeasurementresults,fromimagepre-processing,thresholdadjustments,generationofa3Dbinarymask,definitionofmeasurementparametersuptothegenerationofreports.Alternatively,userscanapply3Dprocessingtoolsinanyorder.Akeyelementoftheprocessingtoolsisthe3DimagecalculatorwiththeABIlitytooperatecompleximageanalysisformulas.
WizardforAutomated3DAnalysis
The3DAnalysiswizardhelpsyouobtainkeydatafromyoursample.Itguidesyouthroughtheanalysisprocessandoffersalargeselectionoffilters,asegmentationtoolwithoptionalautomaticthresholddeterminationandbinaryfilters.3Dobjectsalsocanbecombinedorseparatedmanually.Keydatatobeobtainedarevolume,filledvolume,voxelcount,surfacearea,filledsurfacearea,diameter,manyshapeandintensityparametersaswellasobjectpositionplusstatisticsforallsupportedfeatures.
Interactive3DMeasurements
The3Dinteractivemeasurementtoolwillhelpyouobtainthedatayouneedrangingfromvolume,surface,distance,andangletopolygonoutlines,freehandcurvesandobjectcount.
ProcessingToolbox
Inadditiontothe3DAnalysiswizard,youcantakefullcontrolovertheanalysisprocess:Youcanapplyprocessingtoolsinanyordertoanalyzethe3Dvolume.Processtoolsincludesmoothingandmorphologicalfilters,segmentationtools,generationofbinarymask,andapowerfulimagecalculator.
3DImageCalculator
Theabilitytooperatecompleximageanalysisformulasmakesthe3Dimagecalculatorthekeyelementoftheprocessingtools.Featuresincludearithmetic,booleanandtranscendentaloperations.
