Breakthroughdiscoveriescanhappenwhenyouareintherightplaceattherighttime.LeicaHCSAcanspeeduptheprocessofdiscoverythroughhighcontentscreeningautomation,HCSA.TheintegrationofLeicaHCSAintotheconfocalandwidefieldsystemsofLeicaMicrosystemsallowsyoutostandardizeBIOLOGicalapplicationsforrapidandreproducIBLeresults.
Combinetheflexibilityofapoint-scanningconfocalwiththehighspeedofacamera-basedwidefieldsysteminthesameassaytosavetime.Compensationforspecimendrift,singleobjecttrackingandimmersionfluidaretakencareofforhighqualityrawdata.
LeicaHCSAsupportsallmicroscopeplatformsofLeicaMicrosystems:LeicaTCSSP8,LeicaTCSSPEandLASXWidefieldSystems.
RegardlessofwhichplatformyouchooseLeicaHCSAcanmarkthestartofunderstandingofyourbiologicalquestion,too.
Startthinkingbig
Youcanmakestronginferencesbasedonrobuststatisticsbyscreeningalargenumberofsamplesorconditions.LeicaHCSAsupportsyouwithalltheflexibilityyouneedtoincorporateanystandardsampledishormultiwellplate.Sincedataiswrittentoastoragedevicecontinuouslyimageanalysiscanstartimmediately.UsingOME-TiffformatmaximizescompatibilityandscalABIlity.Tissuesororganstypicallywon’tfitintoonefieldofview.LeicaHCSAoffersapowerfulstitchingsolutioncalledMosaic.Youcancombinetheselargeimageswithtimeseries,multiwellformatsorcustompositions.LeicaHCSAwillhelpyoutotacklebigquestionswhileprovidingthefullflexibilityofaresearchmicroscope.
Startincreasingyourthroughput
Allimageparametersaswellasthegeometryofthesampleareadjustedinadvanceoftheimageacquisitionbyusingscanningtemplates.Thesescanningtemplatesdecoupleassaydevelopmentfromthehighcontentscreentogainprecioustime.Whiletheproductionenvironmentcontinuoustochurnoutresultsnewideascanalreadybetestedofflinebythefacilitymanager.Onceestablishedtheimagingprotocolbecomespartofascanningtemplatereadyfordatarecording.Thescanningtemplatedocumentsalldetailsoftheacquisitionprocesstobeapplieddirectlyaswellasforreviewbytheresearchertoensuremaximumdataquality.
Startdetectingrareevents
ThetrADItionalparadigmofsiftingthroughalargenumberofspecimensmanuallycannotcopewithrareevents.ComputerAidedMicroscopy(CAM)allowstheseeventstobecontinuouslystreamedtoexternalstoragedevicesusingtheOME-TIFFformatwheretheyareanalysedinparalleltoimageacquisition.InconjunctionwithCAM,LeicaHCSAcanrespondtofeedbackfromanalysissoftwareaboutaneventdetectedduringacquisition.Thispowerfulapproachhasproventosimplifylargecollaborativescreeningcampaigns.
