Singlemoleculedetection(SMD)andanalysisisanelegantwaytoexaminedynamicsandinteractionsinsidecellularsystems.ThenewLeicaTCSSMDSeriesperfectlyintegratestheconfocalmicroscopeLeicaTCSSP8andtheSMDspecificsoftwareandhardwarefromPicoQuantintoonesystem.
ThewellprovencomponentsconstituteaflexIBLeplatformforaplethoraoftimeresolvedtechniquesincludingFCS,FCCS,FLIM andFLCS.Theglobalsystemcontrolanditselegantinterfaceopenthedoortoamultitudeofbiophysicaltechniqueswhichprovidequantitativeparametersformodelbuilding.
Globalexperimentcontrolbyfullsystemintegration
Withthefullintegrationofallcomponentstheusercancontrolthecompleteexperimentviaoneinterface.Dedicatedapplicationwizardsprovidereproducibleresultsquicklyandeasily.Thehighdegreeofautomationallowsnewrecordingstrategies.OnlineSMDdatadisplayandinteractivefeedbackgrantsatightcontrolofdataquality.
Spectralandlifetimeinformationatonce–Combinethepower!
ThecombinationofaLeicaTCSSP8XwhitelightlasersystemwithspectralFLIMdetectionresultsinatuneable,filterfreeFLIMsystem.HighestFLIMdataqualityisachievedbytuningboth,excitationanddetectiontotheoptimalspectralrange.UsingtheconvenienceoftheSMDFLIMwizard,automatedFLIMexcitationanddetectionstackscanbeacquired,whichisveryusefultoseparatefluorescenceofmultiplestainedsamplesandtocharacterizenewdyes.
QuickResults-betterdatareliABIlity
TheonlinedisplayofimportantstatisticalcontrolparametersuchasthecorrelationcurveandfastFLIMimage,thecountrate,themolecularbrightnessorthemaximalphotoncountnumberallowthecontinuousevaluationofthedataqualityduringtherunningFCSorFLIMexperiment.SMDdatacanbealreadyinterpretedduringthemeasurementseries.
MaximumflexibilityinSMDanalysis
DuetoauniversalSMDrawdataformattheverysamedatafilecanbeanalyzedindifferentways.Dependingonhardwareandexperimentaldesign,bothcorrelationandlifetimeinformationcanbeextractedfromasinglemeasurementyieldingamaximumofinformationaboutthesample.FCSsystemscanbeupgradedwithFLIMandviceversatoachievethemostflexibleFLCSconfiguration.
