ThewhitelightlasersourceoftheLeicaTCSSP8Xperfectlymatchesthewavelengthofanyfluorophore.Uptoeightexcitationlinescanbeused–simultaneously.
Bytuningbothexcitationanddetection,completeexcitationemissionspectracanbeacquired.
Withthisspectralinformation,anydyecanbeoptimallyexcitedwithminimumcross-excitationandspecimendamage.
Inaddition,thepulsedWhiteLightLaser(WLL)suppliesexcitationlightforfluorescencelifetimemeasurements.
FLIMprovidesinformationaboutthedirectenvironmentofamolecule,likelocalpHorbindingtoanothermolecule.
Completespectralfreedom
Thechoiceoffluorescentlabelsisnotlimitedanylongerbythehardware.TheresearcherselectsthedyesaccordingtotheiravailABIlityorthescientificneeds.Withuptofivespectraldetectionchannels,theLeicaTCSSP8Xiscompletelytunableandfullyadaptstothespecimen.
Fullspectralcharacterizationofimages
Newdyesorfluorescentproteinscanbediscoveredandcharacterized.Atwo-dimensionalexcitation-emissionspectrumcharacterizesthespectralpropertiesofanyfluorescentsample.Fluorophoreswithidenticalemissionorexcitationspectraarediscriminated.Thisisessentialformulti-colorfluorescenceexperiments.
ZerobackgroundbyLightGate
LightGateisaclever,filter-freemethodtoremoveunwantedsignalfromtheimagedata.Anadjustabletimegateswitchesoffthedatacollectionduringthewhitelightlaserpulse.Additionally,non-wantedfluorescencecanberemovedbyadjustingthetimegate.Thiswaythehighestimagecontrastwithoutanyreflectioncontributionisobtained.
FreeselectionofFLIMexcitationwavelength
TheWhiteLightLaserisanappropriateexcitationsourceforfluorescencelifetimemeasurements.ItscontinuoustunabilityallowsperformingFLIMmeasurementatanyexcitationwavelength.Lifetimeoforangeandreddyescannowbeobtained.Theoptimallaserrepetitionrateisselectedviaanintegratedpulsepicker.
