WiththeLeicaPLANAPO2.0xCORRObjective,therefractiveindexcanbeadjusted–thiswayyouwillhavepin-sharpimagesevenifthereisawatercolumnof5mmbetweenyoursampleandtheobjective.Theobjectiveenablesuserstoobserveanddocumentsamplesasifthewaterdidnotexist.
Whenobservingsamplesimmersedinawaterysolution,structurestendtoblurespeciallyinhighmagnification.Thisisduetothedifferentrefractiveindicesofair(index=1)andwater(index=1.3).Potentiallyinterestingorimportantstructuresmaybeeasilymisinterpretedthroughsphericalaberrationsthatmayoccurasregularobjectivesarededicatedtotheusewithsamplessurroundedbyaironly.
Regularobjective
StudyingVascularDevelopmentusingZebrafish(somites).Imagetakenwitharegularobjective.
ImagecourtesyofandtakenbyMailinJ.Hamm,AngiogenesisLaboratory,UniversityofMuenster.
LeicaPLANAPO2.0xCORR
StudyingVascularDevelopmentusingZebrafish(somites).ImagetakenwiththeLeicaPLANAPO2.0xCORRobjective.
ImagecourtesyofandtakenbyMailinJ.Hamm,AngiogenesisLaboratory,UniversityofMuenster.
Regularobjective
StudyingVascularDevelopmentusingZebrafish(eye).Imagetakenwitharegularobjective.
ImagecourtesyofandtakenbyMailinJ.Hamm,AngiogenesisLaboratory,UniversityofMuenster.
LeicaPLANAPO2.0xCORR
StudyingVascularDevelopmentusingZebrafish(eye).ImagetakenwiththeLeicaPLANAPO2.0xCORRobjective.
ImagecourtesyofandtakenbyMailinJ.Hamm,AngiogenesisLaboratory,UniversityofMuenster.
