Zyagen/WIPI1 Antibody/9779/1 Ea

价格
¥6700.00
货号:9779
浏览量:96
品牌:Zyagen
服务
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商品描述

Background

WIPI1Antibody:WIPI1(WDrepeatdomain,phosphoinositideinteracting-1),alsoknownasWIPI1,ATG18orWIPI49,isthoughttoplayaroleinautophagyandmayregulateproteintraffickingincertainrecyclingpathways.ItcontainsthreeWDrepeatsandhasa7-bladedpropellerstructurewithaconservedmotifthatfacilitatesitsinteractionwithotherproteins.WIPI1localizestocytoplasmicvesicles,endosomes,clathrin-coatedvesiclesandthetrans-Golginetwork.Itisubiquitouslyexpressedwithhighestexpressioninheart,testis,placenta,pancreasandskeletalmuscle.WIPI1isupregulatedinavarietyoftumors,suggestingaroleincarcinogenesis.

 

Description
WIPI1 Antibody
WesternblotanalysisofWIPI1inratcolontissuelysatewithWIPI1antibodyat(A)1and(B)2μg/mL.

 

 

 

 

 

 

 

 

 

 

 

Specifications

AdditionalNames
WIPI1Antibody:WDrepeatdomainphosphoinositideinteracting1,ATG18,WD40repeatproteininteractingwithphosphoinositidesof49kDa,WIPI-1,WIPI49
Storage
WIPI1antibodycanbestoredat4ËšCforthreemonthsand-20ËšC,stableforuptooneyear.Aswithallantibodiescareshouldbetakentoavoidrepeatedfreezethawcycles.Antibodiesshouldnotbeexposedtoprolongedhightemperatures.
Reference

  • Proikas-CezanneTandRobenekH.Freeze-fracturereplicaimmunolabellingrevealshumanWIPI-1andWIPI-2asmembraneproteinsofautophagosomes.J.Cell.Mol.Med.2011;15:2007-10.
  • JeffriesTR,DoveSK,MichellRH,etal.PtdIns-specificMPRpathwayassociationofanovelWD40repeatprotein,WIPI49.Mol.Biol.Cell.2004;15:2652-63.
  • Proikas-CezanneT,WaddellS,GaugelA,etal.WIPI-1(WIPI49),amemberofthenovel7-bladedWIPIproteinfamily,isaberrantlyexpressedinhumancancerandislinkedtostarvation-inducedautophagy.Oncogene2004;23:9314-25.
  • Proikas-CezanneT,RuckerbauerS,StierhofYD,etal.HumanWIPI-1puncta-formation:anovelassaytoassessmammalianautophagy.FEBSLett.2007;581:3396-404.]
Formulation
PhysicalState:Liquid 

Concentration:1mg/mL
ProteinGINumber
157388939
ProteinAccessionNumber
NP_060453
MolecularWeight
49kDa
Disclaimer
Thisproductisforresearchuseonly.
TestedApplication
E,WB,IF
BlockingPeptide
Cat.No.ZP-7041-WIPI1Peptide
UserNote
Optimaldilutionsforeachapplicationtobedeterminedbytheresearcher.
Conjugate
None
Purification
WIPI1Antibodyisaffinitychromatographypurifiedviapeptidecolumn.
Clonality/Clone
ThisisapolyclonalWIPI1Antibody.
Isotype
IgG
Host
WIPI1antibodywasraisedinrabbit.

Pleaseuseanti-rabbitsecondaryantibodies.
Specificity
AtleasttwoisoformsofWIPI1areknowntoexist;thisantibodywilldetectbothisoforms.
Immunogen
WIPI1antibodywasraisedagainsta17aminoacidsyntheticpeptidenearthecarboxyterminusofhumanWIPI1.
Application
WIPI1antibodycanbeusedfordetectionofWipi1byWesternblotat1-2μg/mL.Forimmunofluorescencestartat20μg/mL.
Buffer
WIPI1AntibodyissuppliedinPBScontaining0.02%sodiumazide.
SpeciesReactivity
H,M,R

 

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