Background
WIPI1Antibody:WIPI1(WDrepeatdomain,phosphoinositideinteracting-1),alsoknownasWIPI1,ATG18orWIPI49,isthoughttoplayaroleinautophagyandmayregulateproteintraffickingincertainrecyclingpathways.ItcontainsthreeWDrepeatsandhasa7-bladedpropellerstructurewithaconservedmotifthatfacilitatesitsinteractionwithotherproteins.WIPI1localizestocytoplasmicvesicles,endosomes,clathrin-coatedvesiclesandthetrans-Golginetwork.Itisubiquitouslyexpressedwithhighestexpressioninheart,testis,placenta,pancreasandskeletalmuscle.WIPI1isupregulatedinavarietyoftumors,suggestingaroleincarcinogenesis.
Description
WesternblotanalysisofWIPI1inratcolontissuelysatewithWIPI1antibodyat(A)1and(B)2μg/mL.
Specifications
AdditionalNames
WIPI1Antibody:WDrepeatdomainphosphoinositideinteracting1,ATG18,WD40repeatproteininteractingwithphosphoinositidesof49kDa,WIPI-1,WIPI49Storage
WIPI1antibodycanbestoredat4ËšCforthreemonthsand-20ËšC,stableforuptooneyear.Aswithallantibodiescareshouldbetakentoavoidrepeatedfreezethawcycles.Antibodiesshouldnotbeexposedtoprolongedhightemperatures.Reference
- Proikas-CezanneTandRobenekH.Freeze-fracturereplicaimmunolabellingrevealshumanWIPI-1andWIPI-2asmembraneproteinsofautophagosomes.J.Cell.Mol.Med.2011;15:2007-10.
- JeffriesTR,DoveSK,MichellRH,etal.PtdIns-specificMPRpathwayassociationofanovelWD40repeatprotein,WIPI49.Mol.Biol.Cell.2004;15:2652-63.
- Proikas-CezanneT,WaddellS,GaugelA,etal.WIPI-1(WIPI49),amemberofthenovel7-bladedWIPIproteinfamily,isaberrantlyexpressedinhumancancerandislinkedtostarvation-inducedautophagy.Oncogene2004;23:9314-25.
- Proikas-CezanneT,RuckerbauerS,StierhofYD,etal.HumanWIPI-1puncta-formation:anovelassaytoassessmammalianautophagy.FEBSLett.2007;581:3396-404.]
Formulation
PhysicalState:Liquid
Concentration:1mg/mLProteinGINumber
157388939ProteinAccessionNumber
NP_060453MolecularWeight
49kDaDisclaimer
Thisproductisforresearchuseonly.TestedApplication
E,WB,IFBlockingPeptide
Cat.No.ZP-7041-WIPI1PeptideUserNote
Optimaldilutionsforeachapplicationtobedeterminedbytheresearcher.Conjugate
NonePurification
WIPI1Antibodyisaffinitychromatographypurifiedviapeptidecolumn.Clonality/Clone
ThisisapolyclonalWIPI1Antibody.Isotype
IgGHost
WIPI1antibodywasraisedinrabbit.
Pleaseuseanti-rabbitsecondaryantibodies.Specificity
AtleasttwoisoformsofWIPI1areknowntoexist;thisantibodywilldetectbothisoforms.Immunogen
WIPI1antibodywasraisedagainsta17aminoacidsyntheticpeptidenearthecarboxyterminusofhumanWIPI1.Application
WIPI1antibodycanbeusedfordetectionofWipi1byWesternblotat1-2μg/mL.Forimmunofluorescencestartat20μg/mL.Buffer
WIPI1AntibodyissuppliedinPBScontaining0.02%sodiumazide.SpeciesReactivity
H,M,R
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上海起发实验试剂有限公司
2017-01-15 02:09:07
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