Background
FOXO3Antibody:FOXO3isaubiquitouslyexpressed75kDaproteinmemberofasubfamilyoftheforkheadhomeoticgenefamilyoftranscriptionfactorsandshuttlesbetweenthecytoplasmandnucleus.FOXOtranscriptionfactorsarekeyplayersofcellfatedecisions,metabolism,stressresistance,tumorsuppressionandareregulatedbygrowthfactors,oxidativestressornutrientdeprivation.FOXO3isinvolvedwithmTORintheregulationofautophagyinskeletalmuscle,andactivatesproteindegradationinatrophyingmusclecells.FOXO3hasalsobeenimplicatedinseveralneurodegenerativedisordersincludingaging,neuromusculardisease,systemiclupuserythmatosus,strokeanddiabeticcomplications.
Description
WesternblotanalysisofFOXO3inA-20celllysatewithFOXO3antibodyat(A)0.5and(B)1μg/mL.
ImmunocytochemistryofFOXO3inA-20cellswithFOXO3antibodyat4μg/mL.
Specifications
AdditionalNames
FOXO3Antibody:ForkheadboxO3,FOXO2,FOXO3A,FKHRL1,FKHRL1P2Disclaimer
Thisproductisforresearchuseonly.UserNote
Optimaldilutionsforeachapplicationtobedeterminedbytheresearcher.Immunogen
FOXO3antibodywasraisedagainstan18aminoacidsyntheticpeptideneartheaminoterminusofhumanFOXO3.Isotype
IgGClonality/Clone
ThisisapolyclonalFOXO3Antibody.Conjugate
NoneFormulation
PhysicalState:Liquid
Concentration:1mg/mLPurification
FOXO3Antibodyisaffinitychromatographypurifiedviapeptidecolumn.Host
FOXO3antibodywasraisedinrabbit.
Pleaseuseanti-rabbitsecondaryantibodies.Application
FOXO3antibodycanbeusedfordetectionofFOXO3byWesternblotat0.5-1μg/mL.Antibodycanalsobeusedforimmunocytochemistrystartingat4μg/mL.Forimmunofluorescencestartat20μg/mL.TestedApplication
E,WB,ICC,IFBuffer
FOXO3AntibodyissuppliedinPBScontaining0.02%sodiumazide.BlockingPeptide
Cat.No.ZP-5813-FOXO3PeptideStorage
FOXO3antibodycanbestoredat4ËšCforthreemonthsand-20ËšC,stableforuptooneyear.Aswithallantibodiescareshouldbetakentoavoidrepeatedfreezethawcycles.Antibodiesshouldnotbeexposedtoprolongedhightemperatures.SpeciesReactivity
H,M,RProteinGINumber
42519916ProteinAccessionNumber
NP_963853Reference
- AndersonMJ,ViarsCS,CzekayS,etal.CloningandcharacterizationofthreehumanforkheadgenesthatcompriseanFKHR-likegenesubfamily.Genomics1998;47:187-99.
- GreerELandBrunetA.FOXOtranscriptionfactorsattheinterfacebetweenlongevityandtumorsuppression.Oncogene2005;24:7410-25.
- MammucariC,SchiaffinoS,andSandriM.DownstreamofAkt:FoxO3andmTORintheregulationofautophagyinskeletalmuscle.Autophagy2008;4:524-6.
- ZhaoJ,BraultJJ,SchildA,etal.FoxO3coordinatelyactivatesproteindegradationbytheautophagic/lysosomalandproteasomalpathwaysinatrophyingmusclecells.CellMetab.2007;6:472-83.]
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上海起发实验试剂有限公司
2017-01-15 02:09:07
上海市
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Zyagen,Zyagen代理,Zyagen上海代理,Zyagen北京代理,Zyagen上海代理,Zyagen国内代理,Zyagen总代理,Zyagen一级代理
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美国Zyagen Laboratories公司位于加利福尼亚州的生物硅谷圣地亚哥,是一家著名的提供基因表达产品和服务的生物公司。从2000年到现在,在基因表达方面的研究及产品的推广一直处于全球地位。Zyagen Laboratories公司由一批训练有素的高水平的学者与研究人员,同时与发展中国家的科学家在生物制药,生物技术等方面有着广泛的合作。