Zyagen/FOXO1 Antibody/9774/1 Ea

价格
¥6700.00
货号:9774
浏览量:102
品牌:Zyagen
服务
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商品描述

Background

FOXO1Antibody:FOXO1,alsoknownasFKHRorForkHeadinRhaBDomyosarcoma,isa70kDaproteinwhichisaubiquitouslyexpressedmemberofasubfamilyoftheforkheadhomeoticgenefamilyoftranscriptionfactorsandshuttlesbetweenthecytoplasmandnucleus.FOXOtranscriptionfactorsarekeyplayersofcellfatedecisions,metabolism,stressresistance,tumorsuppressionandareregulatedbygrowthfactors,oxidativestressornutrientdeprivation.Ininsulin-responsivetissues,stressornutrientabundancetriggersphosphorylationbyPKB/AKT,blockingnucleartranslocationandactivity.GeneticmutationsinvolvingFOXO1Aareacauseofalveolarrhabdomyosarcoma(RMS2).Recentstudieslinktheanti-tumoractivityofFOXO1andtheprocessofautophagy.

 

Description
FOXO1 Antibody
WesternblotanalysisofFOXO1inHelacelllysatewithFOXO1antibodyat1μg/mL.
FOXO1 Antibody
ImmunocytochemistryofFOXO1inHeLacellswithFOXO1antibodyat4μg/mL.

 

 

 

 

 

 

 

 

 

 

Specifications

AdditionalNames
FOXO1Antibody:ForkheadboxO1,FKH1,FKHR,Forkheadinrhabdomyosarcoma,FOXO1A
Disclaimer
Thisproductisforresearchuseonly.
UserNote
Optimaldilutionsforeachapplicationtobedeterminedbytheresearcher.
Immunogen
FOXO1antibodywasraisedagainsta15aminoacidsyntheticpeptideneartheaminoterminusofhumanFOXO1.
Isotype
IgG
Clonality/Clone
ThisisapolyclonalFOXO1Antibody.
Conjugate
None
Formulation
PhysicalState:Liquid 

Concentration:1mg/mL
Purification
FOXO1Antibodyisaffinitychromatographypurifiedviapeptidecolumn.
Host
FOXO1antibodywasraisedinrabbit.

Pleaseuseanti-rabbitsecondaryantibodies.
Application
FOXO1antibodycanbeusedfordetectionofFOXO1byWesternblotat1μg/mL.Antibodycanalsobeusedforimmunocytochemistrystartingat4μg/mL.Forimmunofluorescencestartat20μg/mL.
TestedApplication
E,WB,ICC,IF
Buffer
FOXO1AntibodyissuppliedinPBScontaining0.02%sodiumazide.
BlockingPeptide
Cat.No.ZP-5811-FOXO1Peptide
Storage
FOXO1antibodycanbestoredat4ËšCforthreemonthsand-20ËšC,stableforuptooneyear.Aswithallantibodiescareshouldbetakentoavoidrepeatedfreezethawcycles.Antibodiesshouldnotbeexposedtoprolongedhightemperatures.
SpeciesReactivity
H,M,R
ProteinGINumber
9257222
ProteinAccessionNumber
NP_002006
Reference 

  • AndersonMJ,ViarsCS,CzekayS,etal.CloningandcharacterizationofthreehumanforkheadgenesthatcompriseanFKHR-likegenesubfamily.Genomics1998;47:187-99.
  • GreerELandBrunetA.FOXOtranscriptionfactorsattheinterfacebetweenlongevityandtumorsuppression.Oncogene2005;24:7410-25.
  • BrunetA,BonniA,ZigmondMJ,etal.Aktpromotescellsurvivalbyphosphorylatingandinhibitingaforkheadtranscriptionfactor.Cell1999;96:857-68.
  • LinardicCM.PAX3-FOXO1fusiongeneinrhabdomyosarcoma.CancerLett.2008;270:10-8.]

 

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