MouseLung mRNA(PolyA+RNA)ispurifiedfromtotalRNAextractedfromfreshlyharvestedtissuesusingclassicalguanidineisothiocyanate–phenol:chloroformextractionmethodandtreatedwithDNasetoremoveresidualDNAcontamination.EachpolyA+RNAsampleisenrichedformRNAtranscriptswithtworoundsofoligo(dT)-cellulosepurification,preciselyquantified,andstoredat-80oC.
QualityControl: TheintegrityofeachRNAsampleisverifiedbydenaturedagarosegelelectrophoresis.ThepurityofRNAisassessedbyspectrophotometer(A260/A280:1.9-2.1). ResidualDNAcontaminationistestedbyPCR.
Applications: RNAisidealforinvestigationofraretranscriptusingqPCRorNorthernblotanalysis,preparation ofCDNAfor RT-PCR/RACEanalyses,andexpressionstandardinmicroarrayapplications.
Packing/shipping:TotalRNAsampleisroutinelyprovidedinRNase-freewaterataconcentrationof0.25mg/mlor0.5mg/mlandshippedondryice.Samplecanalsobeprovidedin1mMsodiumcitrate,0.1mMEDTA,oranyotherRNAstoragebuffer.Samplesareshippedondryice.
MSDSandCertificateofAnalysisinPDFfiles: ContactZyagenTechnicalSupportat zinfo@zyagen.com