TheHistoneH3primaryantibodycanbeusedasaninternalloADIngcontrolfornormalizationandisparticularlyeffectivewhendetectingtargetproteinsinnuclearextracts.
TheexpressionofHistoneH3,oranyhousekeepingprotein(HKP),shouldbevalidatedtoensurethatitsexpressiondoesnotchangeunderexperimentalconditions.
Oncevalidated,HistoneH3primaryantibodiescanbeusedforthedetectionofHistoneH3whenperformingmultiplexWesternblotdetection.
DetectHistoneH3RabbitMonoclonalAntibodywithIRDye®Goatanti-RabbitorIRDyeDonkeyanti-Rabbitsecondaryantibodies.
Otheroptionsforhousekeepingproteinnormalization
ReactivityandSpecificity
HistoneH3antibodyissuppliedin10mMHEPES(pH7.5),150mMNaCl,100µg/mLBSA,50%glycerol,and<0.02% sodium="">0.02%>
Donotaliquottheantibody.
Properties | HistoneH3RabbitMonoclonalAntibody(P/N926-42219) |
---|---|
SpeciesCross-Reactivity | Human,mouse,rat,monkey |
TargetMolecularWeight | 17kDa |
Isotype | RabbitIgG |
Specificity/Sensitivity | DetectsendogenouslevelsoftotalHistoneH3protein(includingisoformsH3.1,H3.2,andH3.3)andHistoneH3variantCENP-A.Doesnotcross-reactwithothercorehistones.Maycross-reactwithbovine,chicken,D.melanogaster,hamster,xenopus,andzebrafish. |
Immunogen | AsyntheticpeptidethatcorrespondstothecarboxyterminusofthehumanhistoneH3protein |
TestedApplications | Westernblot(WB),Immunohistochemistry(IHC),Immunofluorescence(IF),FlowCytometry(F) |
RRID
- P/N926-42219:RRIDAB_2814902