TheCellVueBurgundycelllabelingkitusesproprietarymembranelabelingtechnologytostablyincorporateCellVueBurgundy,afluorescentdyewithlongaliphatictails,intolipidregionsofthecellmembrane1.Thelabelingbufferprovidedwiththekit(DiluentC)isaniso-osmoticaqueoussolutionwhichcontainsnophysiologicsaltsorbuffers,detergents,ororganicsolventsandisdesignedtomaintaincellviABIlitywhilemaximizingdyesolubilityandstainingefficiency.Thelabelingefficiencyofstainingisdependentuponthecelltypebeinglabeledandthemembranesofthecells2,3.
UseNIRFluorescentLabelingforCellTrackingandCellProliferationStudies
CellVueproductshavebeenreportedtobeusefulforshort-terminvitrocellproliferationstudies4,celltrackinginisolatedorganpreparationapplications5,andcelltrackingusingthePearl®SmallAnimalImagingSystemorOdyssey®CLxorOdysseyClassicInfraredImagingSystem6.
KitComponents
- CellVueBurgundydyestock(1vialcontaining0.1mL,1x10-3Minethanol)
- DiluentC(1vialcontaining10mL,sufficientfor5labelingreactions)
ExcitationandEmissionPropertiesofCellVueBurgundyDye
TheexcitationandemissionpropertiesofCellVueBurgundydyearecompatIBLewitharangeofcommerciallyavailablenear-infraredfluorescentplatereaders,flowcytometers,invitroandinvivofluorescentimagingsystems,andconfocalmicroscopes.
DataExampleusingCellVueBurgundyKit
References
- Horan,P.K.,andSlezak,S.E.,Nature,340,167-168(1989).
- Horan,P.K.,etal.,MethodsCellBiol.,33,469-490(1990).
- Poon,R.Y.,etal.InLivingColor:FlowCytometryandCellSortingProtocols,Diamond,R.A.,andDeMaggio,S.(Eds.).p.302-352(Springer-Verlag,NewYork,2000).
- Stewart,C.C.,Woodring,M.L.,Podniesinski,E.andGray,B.D.FlowCytometerintheInfrared:InexpensiveModificationstoaCommercialInstrument.Cytometry,PartA,67A,#2,104-111(2005).
- Al-Mehdi,A-B,etal.IncreasedDepthofCellularImagingintheIntactLungUsingFarRedandNearInfraredFluorescentProbes.Int.J.Biomed.Imag.,Vol2006,ArticleID37470,p1-7.
- Thomas,D.L.,etal.,ExperimentalManipulationsofAfferentImmuneResponsesInfluenceEfferentImmuneResponsestoBrainTumors.CancerImmunol.Immunother.Sep,57(9):1323-33(2008).
CellVueisaregisteredtrademarkofPTIResearch,Inc,usedunderlicense.CellVueproductsaresoldunderlicensefromPTIResearch,Inc..USPatent#8,029,767B2.
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