Glyceraldehyde-3-phosphatedehydrogenase(GAPDH)isaconstitutivelyexpressedhousekeepingprotein(HKP).TheGAPDHprimaryantibodycanbeusedasaninternalloADIngcontrolfornormalization.
TheexpressionofGAPDH,oranyHKP,shouldbevalidatedtoensurethatitsexpressiondoesnotchangeunderexperimentalconditions.
Oncevalidated,GAPDHprimaryantibodiescanbeusedforthedetectionofGAPDHwhenperformingmultiplexWesternblotdetection.
DetectGAPDHRabbitMonoclonalAntibodywithIRDye®Goatanti-RabbitorIRDyeDonkeyanti-Rabbitsecondaryantibodies.
Otheroptionsforhousekeepingproteinnormalization
ReactivityandSpecificity
GAPDHantibodyissuppliedin10mMHEPES(pH7.5),150mMNaCl,100µg/mLBSA,50%glycerol,and<0.02% sodium="">0.02%>
Donotaliquottheantibody.
Properties | GAPDHRabbitMonoclonalAntibody(P/N926-42216) |
---|---|
SpeciesCross-Reactivity | Human,mouse,rat,monkey |
TargetMolecularWeight | 37kDa |
Isotype | RabbitIgG |
Specificity/Sensitivity | DetectsendogenouslevelsoftotalGAPDHprotein.Maycross-reactwithpig |
Immunogen | AsyntheticpeptidethatcorrespondstoresiduesnearthecarboxyterminusofhumanGAPDH |
TestedApplications | Westernblot(WB),Immunohistochemistry(IHC),Immunofluorescence(IF) |
RRID
- P/N926-42216:RRIDAB_2814901