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苏州工业园区生物纳米园A4#216
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4000-520-616 / 18915418616
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0512-67156496
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商品描述
RubiscoQuantitationKit
ProductInformation
KitContains:
- RabbitpolyclonalantibodytoRbcL(100µg)
- Rubisco(RbcL)recombinantproteinstandard(250µl)
- PEBextractionbuffer(3x2ml)(4Xstock)
Storage(AntibodyandStandard)
- lyophilized/reconstitutedat-20°C(6months)or-80°C(longtermstorage/years). Pleaseavoidfreezingandthawingofreconstitutedantibodies. Makealiquotsinstead
Storage(Buffer)
- StableatRTforatleast1month;(shorttermstorage/month)at4°Corlongtermstorage(1year)at20°C.
Background:
- TherubiscoquantitationkitissuitableforquantitationofRubiscoinplantandalgalsamplesusingquantitativeimmunoblotting. Thekitcontainsanti-RbcLantibody,recombinantRbcLproteinandPEBbufferforquantitativeproteinextraction.
ApplicationInformation
- Westernblot1:5,000-10,000withstandardECL
- Expected/apparentMW[kDa]:52.7kDa(ArABIdopsisthaliana),52.5kDa(cyanobacteria),52.3(Chlamydomonasreinhardtii)
- Confirmedreactivity:Arabidopsisthaliana,Chlamydomonasreinhardtii,Cyanophoraparadoxa,Emilianahuxleyi,Euglenagracilis,Gonyaulaxpolyedra,Heterosigmaakashiwo,Micromonaspusila,Porphyrasp.Spinaciaoleracea,SynechococcusPCC7942,Thalassiosirapseudonana
- Predictedreactivity:diandmonocots,conifers,mosses,liverworts,welwitschia,greenalgae,redalge,brownalgae,cryptomonad,cyanobacteriaincludingprochlorophytes,gamma-proeobacteria,beta-proteobacteria,alphaproteobacteria
- Notreactivein:noconfirmedexceptinfrompredictedreactivityknowninthemoment
- RbcLproteinstandard:at0.15pmoles/µl,amountenoughforgenerationofstandardcurvein85assays(standardcurve:0.0625pmoles,0.125pmoles,0.25pmoles)
- PEBextractionbuffer:amountenoughtfor48isolationsofplantmaterial(using500µl1xPEBfor100mgfreshweight)or120isolationsofalgalmaterial(using200µl1xPEBforcellamountscorrespondingto4-10ugtotalchlorophyll)
- Storage:-20°C
- BrandName:bioPLUS
- Size:1Kit
- CatalogNo:30210005-1
