CA-C158 Name Description Caspase8isproventohavesubstrateselectivityforthepeptidesequenceIle-Glu-Thr-Asp(IETD).Thiskituses(Ac-IETD)2-R110asafluorogenicindicatorforcaspase8activity.Cleavageofrhodamine110(R110)peptidesbycaspase8generatesstrongfluorescentR110thatismonitoredattheemissionbetween520nmand530nmwiththeexcitationbetween480nmand500nm.ThisspectralfeaturemakesthekitcompatIBLewiththeFITCfilterset. Application TheEliteTMCaspase8AssayKitprovidesalltheessentialcomponentswithanoptimizedassayprotocol.TheassaycanbereADIlyadaptedforhighthroughputscreenings.Itcanbeusedtoeitherquantifytheactivatedcaspase8activitiesinapoptoticcellsorscreenthecaspase8inhibitors. Size 2plates(96-well) Detection Fluorescencemicroplatereader |
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