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CA-C166 | |
Name | EliteTMCalcium Dye Non-Wash Ratiometric Assay(Fluorescence) |
Description | This kitallows homogeneous measurement of intracellular calcium change caused by activation of G-protein coupled receptors or calcium channels.The ratio of 340/380 nm excitation allows accurate measurements of the intracellular Ca2+ concentration.Measuring by ratio considerably reduces the effects of uneven dye loading, leakage of dye, and photo-bleaching, as well as problems associated with measuring Ca2+ in cells of unequal thickness.This assay involves only one step of dye addition and does not require any washing steps. |
Application | This assay can be conveniently performed in a 96-well or 384-well microtiter-plate format and easily adapted to automation. |
Size | Reagents for 10 plates (96-well or 384-well) |
Detection | Fluorescence microplate reader |