Note: The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name	Histone H4 (Acetyl-Lys12) Antibody
Synonyms	H4F2; HIST; HIST1H4A; Histone H4
Immunogen	The antiserum was produced against synthesized peptide derived from human Histone H4 around the acetylated site of Lys12.
Immunogen Range	Oct-59
Clonality	Polyclonal
Application Dilutions	WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000
Specificity	Histone H4 (Acetyl-Lys12) Antibody detects endogenous levels of total Histone H4 protein only when acetylated at Lys12.
Purification	The antibody was purified from rabbit antiserum by affinity-chromatography using acetylated peptide. The antibody against non-acetylated peptide was removed by chromatography using corresponding non-acetylated peptide.
Physical Form	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability	Stable at -20°C for at least 1 year.
NCBI Gene Symbol	HIST1H4A/HIST1H4B/HIST1H4C/HIST1H4D/HIST1H4E/HIST1H4F/HIST1H4H/HIST1H4I/HIST1H4J/HIST1H4K/HIST1H4L/HIST2H4A/HIST2H4B/HIST4H4
Database Links	Gene ID: 121504/554313/8294/8359/8360/8361/8362/8363/8364/8365/8366/8367/8368/8372 Swiss-Prot #: P62805 OMIM #: 142750/602822/602823/602824/602825/602826/602827/602828/602829/602830/602831/602835 Unigene #: Hs.143080/Hs.247816/Hs.248172/Hs.248178/Hs.248179/Hs.278483/Hs.46423/Hs.528055/Hs.533295/Hs.55468/Hs.591790/Hs.655235/Hs.662174/Hs.706635/Hs.718935
Target Modification	Acetyl
Modification Sites	H:K12 M:K12 R:K12
Application Images
Image 1	Immunofluorescence analysis of HeLa cells, using Histone H4 (Acetyl-Lys12) Antibody. The picture on the right is blocked with the synthesized peptide.
Image 2	Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Histone H4 (Acetyl-Lys12) Antibody. The picture on the right is blocked with the synthesized peptide.
Image 3	Western blot analysis of lysates from COS7 cells, treated with TSA 400nM 24h, using Histone H4 (Acetyl-Lys12) Antibody. The lane on the right is blocked with the synthesized peptide.

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