Note: The product is for research use only, not for use in diagnostic or therapeutic procedures |
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Product Name | PDK1 (Phospho-Ser241) Antibody |
Synonyms | 3-phosphoinositide dependent protein kinase-1; hPDK1; kinase PDK1; mPDK1; PDPK1; PkB kinase; Protein kinase B kinase |
Immunogen | The antiserum was produced against synthesized peptide derived from human PDK1 around the phosphorylation site of Ser241. |
Immunogen Range | 210-259 |
Clonality | Polyclonal |
Application Dilutions | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000 |
Specificity | PDK1 (Phospho-Ser241) Antibody detects endogenous levels of PDK1 only when phosphorylated at Ser241. |
Purification | The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide. |
Physical Form | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Storage/Stability | Stable at -20°C for at least 1 year. |
NCBI Gene Symbol | PDPK1 |
Database Links |
Gene ID: 5170 Swiss-Prot #: O15530 OMIM #: 605213 Unigene #: Hs.459692 |
Target Modification | Phospho |
Modification Sites | H,M,R |
Application Images | |
Image 1 | Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using PDK1 (Phospho-Ser241) Antibody. The picture on the right is blocked with the phospho peptide. |
Image 2 | Western blot analysis of lysates from HeLa cells and NIH/3T3 cells, using PDK1 (Phospho-Ser241) Antibody. The lane on the right is blocked with the phospho peptide. |