TestedSpeciesReactivity:MousePredictedSpeciesReactivity:MouseProductFormat:Liquid.Purifiedantibodysuppliedin1xPBSbufferwith0.09%(w/v)sodiumazideand2%sucrose.Clonality:PolyclonalHost:RabbitApplication:WBReconstitutionandStorage:Forshorttermuse,storeat2-8Cupto1week.Forlongtermstorage,storeat-20Cinsmallaliquotstopreventfreeze-thawcycles.GeneSymbol:HHEXOfficialGeneFullName:hematopoieticallyexpressedhomeoboxNCBIGeneId:15242ProteinName:hematopoietically-expressedhomeoboxproteinHhexSwissprotId:P43120ProteinAccession#:NP_032271.1NucleotideAccession#:NM_008245.3AliasSymbols:Hex,Prh,Hex1,Prhx,Hhex-rs2DescriptionofTarget:RecognizestheDNAsequence5"-ATTAA-3"(Bysimilarity).Transcriptionalrepressor.Mayplayaroleinhematopoieticdifferentiation.Establishesanterioridentityattwolevels;actsearlytoenhancecanonicalWNT-signalingbyrepressingexpressionofTLE4,andactslatertoinhibitNODAL-signalingbydirectlytargetingNODAL.ProteinSize(#AA):271MolecularWeight:29kDaPurification:AffinitypurifiedTissueTool:FindtissuesandcelllinessupportedbyDNAarrayanalysistoexpressHHEX. RNASeq:FindtissuesandcelllinessupportedbyRNA-seqanalysistoexpressHHEX. Immunogen:TheimmunogenisasyntheticpeptidedirectedtowardsthemiddleregionofmouseHHEXPeptideSequence:Syntheticpeptidelocatedwithinthefollowingregion:YLSPPERKRLAKMLQLSERQVKTWFQNRRAKWRRLKQENPQSNKKDALDSConcentration:Batchdependentwithinrange:100ulat0.5-1mg/mlBlockingPeptide:Foranti-HHEX(ARP89428_P050)antibodyisCatalog#AAP89428Datasheets/Manuals:Printabledatasheetforanti-HHEX(ARP89428_P050)antibody
Advanced BioMatrix最畅销的产品是PureCol(5005-100ML)。目前已有上千发表的论文使用这款产品。(Nutragen 5010、fibricol 5133和PureCol的物质成分一样,仅仅是浓度不同)浓度越高,胶原蛋白硬度越强。
许多科研人员正从Matrigel产品使用习惯转换过来,下面简单比较下Advanced BioMatrix公司的PureCol和Matrigel:
Matrigel
PureCol
浓度不均一
均一且浓度精确
鼠,肿瘤来源
牛,非肿瘤来源
未知组分
已知且明确的组分
需在冰上分装操作
简单易用
客户可从上表对比Matrigel和VitroCol (人I型胶原蛋白),以人源简单代替牛源。
PureCol® EZ Gel--3D胶原凝胶让实验更简单
含牛源I型胶原蛋白(5 mg/ml)的DMEM/F-12 培养基, 并添加谷氨酰胺,中性pH。