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蚂蚁淘在线 / 品牌中心 / Advanced BioMatrix / Advanced BioMatrix/Poly-L-Ornithine//5172-5MG

Advanced BioMatrix/Poly-L-Ornithine//5172-5MG

价格
￥1500.00
货号：5172-5MG
浏览量：127
品牌：Advanced BioMatrix
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商品描述

ProductDescription

Poly-L-Ornithineisasyntheticaminoacidchainthatispositivelychargedandwidelyusedasacoatingtoenhancecellattachmentandadhesiontobothplasticwareandglasssurfaces.

ThemolecularweightofPoly-L-Ornithinecanvarysignificantlywithlowermolecularweight(30,000Da)beinglessviscousandhighermolecularweight(>300,000Da)havingmorebindingsitespermolecule.Thisproduct’smolecularweightrangesfrom70,000to150,000Da.

Poly-L-Ornithineissuppliedina50mlamberbottlecontaining5mgofPoly-L-Ornithine.Theproducthasbeensterilizedandisready-to-useafterproperadditionofcellculturewater.

Parameter,Testing,andMethodPoly-L-Ornithine#5172
FormPowder
PackageSize5mg
StorageTemperature-20°C
SterilizationMethodIrrADIation
MolecularWeight70,000-150,000Da
Sterility-USPmodifiedNoGrowth
CellAttachmentAssayPass
SourceSynthetic
ShelfLifeMinimumof6monthsfromdateofreceipt

DirectionsforUse

DownloadthefullPDFversionorcontinuereadingbelow:

Usetheserecommendationsasguidelinestodeterminetheoptimalcoatingconditionsforyourculturesystem.Tomaintainsterility,performalloperationsinalaminarflowhood.

  1. Atypicalworkingconcentrationis0.1mg/ml.Toachievea0.1mg/mlconcentration,add50mloftissueculturegradewaterorPBStothe50mlbottle.Ifadifferentconcentrationisdesired,transferdesiredvolumeofsolutionfromthebottletoadilutionvessel.DilutetodesiredconcentrationusingtissueculturegradewaterorPBS.
  2. Addappropriateamountofdilutedmaterialtoculturesurface.Typically,1mlper25cm2isused.Rockgentlytoensureuniformcoatingofculturesurface.

OptionA:

  1. Allowtoincubatefor60minutesatroomtemperature,removeexcesssolutionbyaspiration.
  2. Thoroughlyrinsesurfacewithtissueculturegradewaterandaspiraterinsewater.
  3. Incubateandallowtodryatroomtemperatureor37°C,covered,foratleast2hours.Coatedculturewarecanbestoredforupto1week.GotoStep9

OptionB:

  1. Allowtoincubateovernightatroomtemperature,removeexcesssolutionbyaspiration.
  2. Thoroughlyrinsesurfacewithtissueculturegradewaterandaspiraterinsewater.
  3. Incubateandallowtodryatroomtemperatureor37°C,covered,foratleast2hours.Coatedculturewarecanbestoredforupto1week.
  4. Introducemediumandcellstotheculturesurface.

StoreremainingPoly-L-Ornithinesolutionat-10to30°C

ProductReferences

ReferencesforPoly-L-Ornithine:

Chen,EricYT,etal."ActivatedcharcoalcompositeBiomaterialpromoteshumanembryonicstemcelldifferentiationtowardneuronallineage."JournalofBiomedicalMaterialsResearchPartA100.8(2012):2006-2017.

Boularaoui,SelwaMokhtar,etal."Efficienttransdifferentiationofhumandermalfibroblastsintoskeletalmuscle."Journaloftissueengineeringandregenerativemedicine12.2(2018):e918-e936.

Ergin,Ekin,etal."Time-resolvedfluorescenceresonanceenergytransfer[TR-FRET]assaysforbiochemicalprocesses."Currentpharmaceuticalbiotechnology17.14(2016):1222-1230.

ProductCertificateofAnalysis

Noresultfor.

ProductVideos

link to library blog - Coating a Glass Coverslip with Collagen
CoatingaGlassCoverslipwithCollagen

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SafetyandDocumentation

SafetyDataSheet

CertificateofOrigin

ProductDisclaimer

ThisproductisforR&Duseonlyandisnotintendedforhumanorotheruses.PleaseconsulttheMaterialSafetyDataSheetforinformationregardinghazardsandsafehandlingpractices.

Advanced BioMatrix最畅销的产品是PureCol（5005-100ML）。目前已有上千发表的论文使用这款产品。（Nutragen 5010、fibricol 5133和PureCol的物质成分一样，仅仅是浓度不同）浓度越高，胶原蛋白硬度越强。   许多科研人员正从Matrigel产品使用习惯转换过来，下面简单比较下Advanced BioMatrix公司的PureCol和Matrigel： Matrigel PureCol 浓度不均一 均一且浓度精确 鼠，肿瘤来源 牛，非肿瘤来源 未知组分 已知且明确的组分 需在冰上分装操作 简单易用 客户可从上表对比Matrigel和VitroCol （人I型胶原蛋白），以人源简单代替牛源。 PureCol® EZ Gel--3D胶原凝胶让实验更简单 含牛源I型胶原蛋白（5 mg/ml）的DMEM/F-12 培养基, 并添加谷氨酰胺，中性pH。