Product Description
Poly-L-Ornithineis a synthetic amino acid chain that is positively charged having one hydrobromide per unit of ornithine.Poly-L-Ornithineis widely used as a coating to enhance cell attachment and adhesion to both plasticware and glass surfaces.
The molecular weight ofPoly-L-Ornithinecan vary significantly with lower molecular weight (30,000 Da) being less viscous and higher molecular weight (>300,000 Da) having more binding sites per molecule. This product’s molecular weight ranges from 70,000 to 150,000 Da yielding a solution viscosity for easy handling while providing sufficient binding sites for cell attachment.
Poly-L-Ornithinesurface coatings are designed to improve cell attachment, growth and differentiation of many cell types.
Coated surfaces will often improve cell attachment in reduced or serum-free conditions. This product is supplied in a sterile 50 ml package size at a concentration of 0.1 mg/ml.
| Parameter, Testing, and Method | Poly-L-Ornithine #5058 |
| Form | Solution |
| Package Size | 50 mL |
| Storage Temperature | 2-10°C |
| Sterilization Method | Filtration |
| Molecular Weight | 70,000 - 150,000 Da |
| Sterility - USP modified | No Growth |
| Cell Attachment Assay | Pass |
| Source | Synthetic |
| Shelf Life | Minimum of 6 months from date of receipt |
| Concentration | 0.1 mg/mL |
Directions for Use
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Use these recommendations as guidelines todetermine the optimal coating conditions for your culture system. To maintain sterility, perform all operations in a laminar flow hood.
- A typical working concentration is 0.1 mg/ml. If a different concentration is desired, transfer desired volume of solution from the bottle to a dilution vessel. Dilute to desired concentration using tissue culture grade water or PBS.
- Add appropriate amount of diluted material to culture surface. Typically, 1 mL per 25 cm2is used. Rock gently to ensure uniform coating of culture surface.
- After 5 minutes, remove excess solution by aspiration.
- Thoroughly rinse surface with tissue culture grade water.
- Incubate and allow to dry at room temperature or 37°C, covered, for at least 2 hours.
- Introduce medium and cells to the culture surface.
- Store remaining Poly-L-Ornithine at 2 to 10°C.
Product References
References for Poly-L-Ornithine:
Chen, Eric YT, et al. "Activated charcoal composite biomaterial promotes human embryonic stem cell differentiation toward neuronal lineage."Journal of Biomedical Materials Research Part A100.8 (2012): 2006-2017.
Boularaoui, Selwa Mokhtar, et al. "Efficient transdifferentiation of human dermal fibroblasts into skeletal muscle."Journal of tissue engineering and regenerative medicine12.2 (2018): e918-e936.
Ergin, Ekin, et al. "Time-resolved fluorescence resonance energy transfer [TR-FRET] assays for biochemical processes."Current pharmaceutical biotechnology17.14 (2016): 1222-1230.
Product Certificate of Analysis
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Safety and Documentation
Safety Data Sheet
Certificate of Origin
Product Disclaimer
This product is for R&D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.
