Product Description
Poly-D-Lysine is a synthetic amino acid chain that is positively charged and widely used as a coating to enhance cell attachment and adhesion to both plasticware and glass surfaces.
The molecular weight of Poly-D-Lysine can vary significantly with lower molecular weight (30,000 Da) being less viscous and higher molecular weight (>300,000 Da) having more binding sites per molecule. This product’s molecular weight ranges from 70,000 to 150,000 Da.
Poly-D-Lysine is supplied in a 50 ml amber bottle containing 5 mg of Poly-D-Lysine. The product has been sterilized and is ready-to-use after proper addition of cell culture water.
| Parameter, Testing, and Method | Poly-D-Lysine #5174 |
| Form | Powder |
| Package Size | 5 mg |
| Storage Temperature | -20°C |
| Sterilization Method | Irradiation |
| Molecular Weight | 70,000 - 150,000 Da |
| Sterility - USP modified | No Growth |
| Cell Attachment Assay | Pass |
| Source | Synthetic |
| Shelf Life | Minimum of 6 months from date of receipt |
Directions for Use
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Use these recommendations as guidelines to determine the optimal coating conditions for your culture system. To maintain sterility, perform all operations in a laminar flow hood.
- A typical working concentration is 0.1 mg/ml. To achieve a 0.1 mg/ml concentration, add 50 ml of tissue culture grade water or PBS to the 50 ml bottle. If a different concentration is desired, transfer desired volume of solution from the bottle to a dilution vessel. Dilute to desired concentration using tissue culture grade water or PBS.
- Add appropriate amount of diluted material to culture surface. Typically, 1 ml per 25 cm2is used. Rock gently to ensure uniform coating of culture surface.
Option A:
Allow to incubate for 60 minutes at room temperature, remove excess solution by aspiration.
- Thoroughly rinse surface with tissue culture grade water and aspirate rinse water.
- Incubate and allow to dry at room temperature or 37°C, covered, for at least 2 hours. Coated cultureware can be stored for up to 1 week. Go to Step 9
Option B:
- Allow to incubate overnight at room temperature, remove excess solution by aspiration.
- Thoroughly rinse surface with tissue culture grade water and aspirate rinse water.
- Incubate and allow to dry at room temperature or 37°C, covered, for at least 2 hours. Coated cultureware can be stored for up to 1 week.
- Introduce medium and cells to the culture surface.
Store remaining Poly-D-Lysine solution at -10 to 30°C
Product References
References for Poly-D-Lysine:
Albuquerque, Cristóvão, et al. "Preparation of coverslips for neuronal cultures."Cold Spring Harbor Protocols2009.8 (2009): pdb-prot5272.
Unsain, Nicolas, et al. "Production and isolation of axons from sensory neurons for biochemical analysis using porous filters."JoVE (Journal of Visualized Experiments)89 (2014): e51795.
Li, Ling, Mizuho Fukunaga-Kalabis, and Meenhard Herlyn. "Isolation, characterization, and differentiation of human multipotent dermal stem cells."Skin Stem Cells. Humana Press, Totowa, NJ, 2013. 235-246.
Baik, Matthew, et al. "Identification of invadopodia by TKS5 staining in human cancer lines and patient tumor samples."MethodsX6 (2019): 718-726.
Tammia, Markus, et al. "Egr2 overexpression in Schwann cells increases myelination frequency in vitro."Heliyon4.11 (2018): e00982.
Boularaoui, Selwa Mokhtar, et al. "Efficient transdifferentiation of human dermal fibroblasts into skeletal muscle."Journal of tissue engineering and regenerative medicine12.2 (2018): e918-e936.
Johnstone, Aaron D., et al. "Developmental axon degeneration requires TRPV1-dependent Ca2+ influx."eNeuro6.1 (2019).
Product Certificate of Analysis
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Safety and Documentation
Safety Data Sheet
Certificate of Origin
Product Disclaimer
This product is for R&D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.
