ProductDescription
ECMSelect®ArrayKitUltra-36isanextracellularmatrixscreeningarray.Thisproducthasthirtysix(36)humanextracellularmatrixprotein(ECM)conditionsthataredepositedontothehydrogelsurfaceasprintedarrayspots.EachECMconditionisprintedwithnine(9)replicatespotswhereeachspothasdiameterof400µm.TheECMsarelocalizedwithineachspotwithoutcrossdiffusionfromneighboringspots,thereforeeachspotrepresentsanindependent‘well’orexperiment.
TheECMSelect®ArrayKitUltra-36containsamicroscopeglassslideprintedwiththeECMconditionscontainedwithinaprotectiveslideholderandapre-sterilizedtray.Thecomponentsaresterilizedandready-to-use.
CellsofinterestareseededontotheECMSelect®Arrayslideandallowedtobeculturedintheincubatorforthedesiredamountoftime.Cellmorphology,attachmentandgrowthcanbevisualizedusingabrightfieldmicroscope.SpecificcellularbehaviorscanbemonitoredbystainingthecellsontheslideusingspecificfluorescencebasedMarker.Fluorescencesignalcanbedetectedusingfluorescencemicroscopeimagingsystem.
TheECMSelect®ArrayKitUltra-36issimpletouseincomparisontoperformingthesameexperimentinmulti-welltissuecultureplates.Cellseeding,fixing,washingandprocessstepsaredoneinonesinglesolutionexchange,eliminatingmulti-wellsolutionhandlings.Fromstarttofinish,theoptimalECMforculturingcellscanbedeterminedinlessthan24hoursformostcelltypes.
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ProductQ&A
Becauseitiscontactprinting,itishardtodeterminethethepreciseamount.Basedonproteinstainingresult,eachspotisabout0.1-1ng.
TheremaybepossibilityofantagoNISTeffectoftwodifferentcomponent.Wehavenotseenthisveryoften,whatweusuallyseeisoneofthecomponentbyitselfdoesnotprovideadhesioneffect,butwhencombinewithanotherECM,greatlyenhancetheotherECM.
Thestiffnessis~10kPa.
MountingmediatendstodestroythepolyacrylamidehydrogelthattheECMspotsareprintedonto.Thebestmethodwehavefoundistoincubatetheentireslidefor20minuteswith4%PFApriortothestainprotocol.Theslidewascover-slippedwitha90%glycerolmediumcontainingthePPDanti-fadecompoundandsealedwithfingernailpolisharoundtheedges.
ProductReferences
ReferencesforECMSelect®:
Shaheen,NoelL.,etal."Extracellularmatrixcompositionmodulatesangiosarcomacellattachmentandproliferation."Oncoscience11-12(2017):178.
Ferreira,L.P.,V.M.Gaspar,andJ.F.Mano."DesignofSphericallyStructured3DInvitroTumorModels-AdvancesandProspects."ActaBiomaterialia(2018).
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ProductDisclaimer
ThisproductisforR&Duseonlyandisnotintendedforhumanorotheruses.PleaseconsulttheMaterialSafetyDataSheetforinformationregardinghazardsandsafehandlingpractices.
