Product Description
Bovine Collagen Type II is one of a family of proteins found particularly in the flesh and connective tissues of mammals (approximately one-third of the body’s total protein). Over two dozen types of collagen have been described; Type II is found predominantly in hyaline cartilage (50% of the protein in hyaline is collagen type II) and is also found in the vitreous humor of the eye.
The product is supplied as a sterile solution at approximately 0.5 mg per vial at approximately ~.50 mg/ml in 500 mM acetic acid. Type II collagen is provided in user-friendly packaging for use and storage. Type II collagen may be used to culture a variety of cell types. Type II collagen is typically used as a coating material for cell culture studies, for the formation of 3D collagen gels, or as an antigen for antibody production.
| Parameter, Testing, and Method | Type II Collagen#5196 |
| Sterilization Method | Filtration |
| Form | Solution |
| Package Size | 0.5 mg (1 mL) |
| Storage Temperature | 2-10°C |
Collagen Concentration - Biuret | ~0.5mg/mL |
Shelf Life | Minimum of 6 months from date of receipt |
| pH | ~2.5 |
Electrophoretic Pattern - Coomassie Blue | Characteristic |
| Endotoxin (LAL) | <5.0 EU/mL |
| Cell Attachment Assay | Pass |
| Source | BovineCartilage |
Directions for Use
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Coating Procedure
Note:Use these recommendations as guidelines todetermine the optimal coating conditions for your culturesystem.
- Remove required quantity of collagen from the bottle and dispense into a dilution vessel.
- Dilute the product with a 500 mM acetic acid solution to desired concentration.
- Swirl contents gently until thoroughly mixed.
- Add approximate amount of diluted Type II Collagen to the culture surface ensuring the entire surface is coated.
- Incubate at room temperature, covered for 1-2 hours.
- After incubation, aspirate any excess remaining material.
- Rinse coated surface carefully with a sterile medium or balanced salt solution. Avoid scratching surfaces.
- Aspirate remaining material from coated surface.
- Coated culture vessels are now ready to use. The coated culture vessels may be stored at 2 to 10°C
Product Certificate of Analysis
Product Videos
Coating Plates with Collagen
Video
Coating a Glass Coverslip with Collagen
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Safety and Documentation
Safety Data Sheet
Certificate of Origin
Product Disclaimer
This product is for R&D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.
