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公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
Description
TheThermoScientific™Pierce™QuantitativeColorimetricPeptideAssayisaneasy-to-usecolorimetricmicroplateassaydesignedspecificallytoimprovethesensitivityandreproducibilityofquantitationofpeptidemixtures.•Sensitive—accuratelydetectaslittleas25µg/mLofpeptidemixture
•Robust—assayperformancerigorouslytestedusingpeptidedigestmixtures
•Robustpeptidedigeststandard—kitincludesavalidatedpeptidedigeststandardforimprovedreproducibilityofquantitation
•CompatIBLe—workswithmanyreagents,includingmassspectrometrysamplepreparationreagentsandTMT™-labeledpeptides
•Convenient—easymix-andread-formatandstablecolorimetricsignalthatmaybereadinaslittleas15minutes
ThePierceQuantitativeColorimetricPeptideAssayprovidesmodifiedBCAreagentsforthereductionofCu+2toCu+1andaproprietarychelatorthathavebeenoptimizedforthequantitationofpeptidemixtures.Inthisreaction,thecopperisfirstreducedbytheamidebackboneofpeptidesunderalkalineconditions(Biuretreaction).Theproprietarychelatorthencoupleswiththereducedcoppertoformabrightredcomplexwithabsorbanceof480nm.Thesignalproducedfromthisreactionis3-4foldmoresensitivethantheMicro-BCAProteinAssayforpeptideanalysis.
Thekitalsocontainsahighqualitypeptidedigestreferencestandardforuseingeneratinglinearstandardcurvesandtoserveasacalibrationcontrol.Thiscolorimetricpeptideassayrequiresasmallamountofsample(20µL)andhasaworkingpeptideconcentrationrangeof25–1000μg/mL.Theincreasedsensitivity,lowsampleassayvolume,andincludedreferencestandardareessentialforaccurateandrobustmeasurementofpeptidedigestsamples,especiallyformassspectrometryapplications.
Applications:
•Recommendedforpeptidemixtures
•Notrecommendedforsyntheticpeptidesbecausetheassayisaffectedbypeptideaminoacidcontent
•Normalizingsampleconcentrationsforquantitativedownstreamapplications
•NormalizingsampleloADIngvolumesforLC,MS,andLC/MSapplications
•Measuringrecoveryafterpeptideclean-upprocedures
ForResearchUseOnly.Notforuseindiagnosticprocedures.
Figures
SensitivityofPierceQuantitativeFluorometricPeptideAssay
ImprovedSensitivityofPierceQuantitativeColorimetricPeptideAssay
Colorimetricassaychemicalcompatibility
Quantitationcomparisonbetweencolorimetricandfluorometricpeptideassays
Specifications
| DetectionMethod: | Colorimetric |
|---|---|
| FinalProduct: | Peptide |
| ForUseWith(Equipment): | MicroplateReader |
| ProductLine: | Pierce™ |
| ProductSize: | 500assays |
| StartingMaterial: | Peptides, Protease-digestedprotein |
| WorkflowStep: | In-ProcessTesting |
Contents&storage
ColorimettricAssayReagentA,50mL
ColorimetricAssayReagentB,2x25mL
ColorimetricAssayReagentC,2mL
PeptideDigestAssayStandard,1.5mL
Storeinrefrigerator(2–8°C).
ColorimetricAssayReagentB,2x25mL
ColorimetricAssayReagentC,2mL
PeptideDigestAssayStandard,1.5mL
Storeinrefrigerator(2–8°C).
