Description

ThermoScientificPageRulerPlusPrestainedProteinLadderisamixtureofnine(9)blue-,orange-andgreen-stainedproteins(10to250kDa)foruseassizestandardsinproteinelectrophoresis(SDS-PAGE)andWesternblotting.

FeaturesofPageRulerPlusPrestainedProteinLadder:

•Sizerange—nineproteinsspanning10to250kDa
•Ready-to-use—suppliedinaloADIngbufferfordirectloadingongels;noneedtoboil
•Sharpbands—color-codedbandsofsimilarintesityforeasyvisualization
•Qualitytested—eachlotevaluatedbySDS-PAGEandWesternblotting
•Brightreferencebands—orangeat70and25kDa,andgreenat10kDa
•Membrane-compatIBLe—coloredbandstransfertomembranesforWesternblotting

ThisprestainedproteinMWMarkerisdesignedformonitoringtheprogressofSDS-polyacrylamidegelelectrophoresis,forassessingtransferefficiencyontoPVDF,nylonandnitrocellulosemembranes,andforestimatingtheapproximatesizeofseparatedproteinsthathavebeenmadevisiblewithgelstainsorWesternblotdetectionreagents.Abluechromophoreisboundtoallproteins,exceptproteinsoftworeferencebandsof70kDaand25kDathatarecoloredwithanorangedyeandonegreenreferencebandof10kDa.PageRulerPlusPrestainedProteinLadderisreadytouse:noheating,furtherdilutionoradditionofareducingagentisrequiredbeforeloadingontoagel.

Includes:
•Dye-stainedproteinsin62.5mMTris-H3PO4(pH7.5at25°C),1mMEDTA,2%SDS,10mMDTT,1mMNaN3and33%glycerol.

Applications:
•MonitoringproteinmigrationduringSDS-polyacrylamidegelelectrophoresis
•MonitoringproteintransferontomembranesafterWesternblotting
•SizingofproteinsonSDS-polyacrylamidegelsandWesternblots