Description

ThermoScientificPageRulerPrestainedProteinLadderisamixtureoften(10)blue-,orange-andgreen-stainedproteins(10to180kDa)foruseassizestandardsinproteinelectrophoresis(SDS-PAGE)andWesternblotting.

FeaturesofPageRulerPrestainedProteinLadder:

•Sizerange—10proteinsspanning10to180kDa
•Ready-to-use—suppliedinaloADIngbufferfordirectloadingongels;noneedtoboil
•Sharpbands—color-codedbandsofsimilarintensityforeasyvisualization
•Qualitytested—eachlotevaluatedbySDS-PAGEandWesternblotting
•Tworeferencebands—orangeat70kDaandgreenat10kDa
•Membrane-compatIBLe—coloredbandstransfertomembranesforWesternblotting

ThisprestainedproteinMWMarkerisdesignedformonitoringtheprogressofSDS-polyacrylamidegelelectrophoresis,forassessingtransferefficiencyontoPVDF,nylonandnitrocellulosemembranes,andforestimatingtheapproximatesizeofseparatedproteinsthathavebeenmadevisiblewithgelstainsorWesternblotdetectionreagents.Theladdercontainsoneorangereferencebandat70kDaandonegreenbandat10kDa.

Includes:
•Dye-stainedproteinsin62.5mMTris-H3PO4(pH7.5at25°C),1mMEDTA,2%SDS,10mMDTT,1mMNaN3and33%glycerol.

Applications:
•MonitoringproteinmigrationduringSDS-polyacrylamidegelelectrophoresis
•MonitoringproteintransferontomembranesafterWesternblotting
•SizingofproteinsonSDS-polyacrylamidegelsandWesternblots