Description

FreeStyle™293ExpressionMediumisananimalorigin–free,chemicallydefined,protein-freemediumspecificallydevelopedtosupportthegrowthandtransfectionof293-FcellsinsUSPensionwithoutadaptation.Themediumiscompleteandready-to-use,withnosupplementationrequired.Gibco®FreeStyle™293ExpressionMediumfeatures:

•Completeandready-to-useformatcontainingGlutaMAX™supplement
•ABIlitytosupportsuspensiongrowthandtransfectionof293-Fcells
•Animalorigin–free,protein-freeandchemicallydefinedformulation
•EasyusewiththeFreeStyle™293Expressionsystems
•Scalabilityinspinnerflasksandbioreactors

Completeandready-to-useformatcontainingGlutaMAX™supplement
Gibco®FreeStyle™293ExpressionMediumisreadytouse.Itdoesnotrequiretheadditionofserum,glutamine,orsurfactants.Gibco®FreeStyle™293ExpressionMediumcontainsGlutaMAX™supplementtominimizetoxicammoniabuild-upandimprovecellviabilityandgrowthinaneasy-to-useformat.

Abilitytosupportsuspensiongrowthandtransfectionof293-Fcells
Gibco®293-F,293-H,andFreeStyle™293-FcellsinsuspensioncanbecultureddirectlyintoGibco®FreeStyle™293ExpressionMediumwithoutadaptation.Cellsadaptedtoothercommerciallyavailableserum-freemediacanbesubcultureddirectlyintoGibco®FreeStyle™293ExpressionMedium,usuallywithoutanyfurtheradaptation.Cellsusuallyrequireadaptationfromserum-containingformulations.CellsculturedinGibco®FreeStyle™293ExpressionMediumcanbetransfectedusinglipidbasedtransfectionreagents,suchas293fectin™reagentandFreestyle™MAXreagent.

Animalorigin–free,protein-freeandchemicallydefinedformulation
Gibco®FreeStyle™293ExpressionMediumisanimalorigin–free,protein-freeandchemicallydefined,allowingforeasierpurificationofyourproteinofinterest.Gibco®chemicallydefinedmediacontainnoproteins,hydrolysates,orcomponentsofunknowncomposition.

EasyuseaspartoftheFreeStyle™293ExpressionSystemandFreeStyle™MAXExpressionSystemforproteinproduction
TheFreeStyle™293ExpressionSystemisacomplete,suspensioncellculturesystemforgeneratinglargeamountsofmammalianrecombinantprotein(seefigure).TheFreeStyle™293ExpressionSystemcombinesGibco®FreeStyle™293ExpressionMediumwith293fectin™reagent,acationic-lipidformulationdesignedtotransfectsuspensionFreeStyle™293-Fcellsathighefficiency.TheFreeStyle™MAXExpressionSystemisabreakthroughtechnologyforrapidandhigh-yieldmammalianproteinproduction.TheFreeStyle™MAXExpressionSystemcombinesGibco®FreeStyle™Media,FreeStyle™MAXReagent,andeitherFreeStyle™CHO-ScellsorFreeStyle™293-Fcells.

Scalabilityinspinnerflasksandbioreactors
ProteinproductionusingGibco®FreeStyle™293ExpressionMediumcanbescaledupinspinnerflasksorbioreactors.Theappropriatespinnerorimpellerspeedandseedingdensityshouldbeoptimizedforeachsystem.Dependingontheimpellerdesignandspeed,itmaybenecessarytosupplementGibco®FreeStyle™293ExpressionMediumwithadditionalPluronic®F-68toavoidsheerstressintheculture.

Universalbagpackaging
Gibco®universalbagsofferflexIBLepackagingthatallowsyoutointegrateasingle-use,disposablelargevolumemediacontainerwithawidevarietyofautomatedcellcultureinstruments,disposablebioreactorsystems,andsterileconnectionprocesses,minimizingtheneedforcostlycustomization.

Productuse
CustomersusingGibco®FreeStyle™293ExpressionMediuminamanufacturingprocess,whohaveasubmissionwiththeFDA,mayrequestaletterofauthorizationfromustoreferenceourTypeIIDrugMasterFile(DMF).

cGMPmanufacturingandqualitysystem
Gibco®FreeStyle™293ExpressionMediumismanufacturedatacGMP-compliantfacilitylocatedinGrandIsland,NewYork.ThefacilityisregisteredwiththeFDAasamedicaldevicemanufacturerandiscertifiedtoISO13485standards.