品牌分类
品牌咨询
联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
Description
Lipofectamine®LTXReagentisaplasmidtransfectionreagentthatofferstherightbalanceofpotencyandgentlenessforyourcells,resultinginthehighestpossIBLetransfectionefficienciesandviABIlities.Lipofectamine®LTXReagentisanadvancedsolutionforgeneexpressioninbothcommonandchallengingcelltypes.NootherplasmidDNA-specifictransfectionreagentcanmatchtheefficiency,convenience,andgentlenessofLipofectamine®LTXReagentfortransfectionofprimary,challenging,andsensitivecelllines.Lipofectamine®LTXReagentissynthesizedfrom100%animalorigin-freecomponents,makingiteasytovalidatetheabsenceofzoonoticdiseases,suchasBSEorviruses,inresearchexperimentsorcelllines.Lipofectamine®LTXReagentoffers:
•Superiordelivery—transfectabroadrangeofcelltypes,includingprimary,challenging,andsensitivecells,withhighefficiency
•Superiorperformance—combinehightransfectionefficiencywithmaximal(>90%)cellviability
•Optimizedprotocols—rapid,simpleprotocolsprovidedformostcelllines
•Ahighlyeffectivereagenttogeneratestablecelllines
Transfectprimarycellsefficiently
Primarycellsareoftendifficulttotransfect.Lipofectamine®LTXReagent,whencombinedwithPLUS™Reagent,providesthehighestleveloftransfectioninprimarycellsofanylipid-orpolymer-basedreagent.ManyprimarycelltypeshavebeensuccessfullytransfectedwithLipofectamine®LTXReagent,includingprimaryneuralProgenitorcells.
Superiordeliveryintochallengingcells
WhenitcomestoachievinghighlevelsofproteinexpressioninRAW267.3macrophages,Lipofectamine®LTXReagentoutperformscompetingtransfectionreagents(seefigure).Achievinghigh-leveltransgeneexpressionincellsthataretrADItionallyrefractorytotransfectionallowsyoutoobtainresultsthatwerepreviouslyunattainable.
Broadcompatibilitywithcelllines
Inadditiontobeingexcellentfortransfectingprimaryandchallengingcells,Lipofectamine®LTXReagentprovidesyouwiththemostversatileapproachtotransfectingestablishedcellslines,includingthosethataresensitivetoothertransfectionreagents.UseourTransfectionSelectionTooltohelpyouselectthebesttransfectionconditionsforthecellswithwhichyouwork.
Stabletransfection
Lipofectamine®LTXstrikestherightbalancebetweenpotencyandgentleness,givingyouthehighestpossiblepercentageofsuccessfullytransfectedcells.ThismakesLipofectamine®LTXReagentthequickestandeasiestreagentforestablishingstabletransfectantsfromchallengingorrefractorycelltypes.
Simple,rapid,serum-compatibleprotocol
Lipofectamine®LTXReagentoffersastreamlinedprotocol—noneedtoremovetransfectioncomplexesorchange/addmediumfollowingtransfection.Asimpleoptimizationprotocolhelpsyougetthebestresultsquickly,soyou"llspendlesshands-ontime,andgetfasterresults.
ForResearchUseOnly.Notforuseindiagnosticprocedures.
Figures
ExamplesofLipofectamine®LTXtransfection
Lipofectamine®LTXtransfectionefficiency
Lipofectamine®LTXReagentcombineshightransfectionefficiencywithlowcytotoxicitysuperiortoFuGENE®6andFuGENE®HDtransfectionreagents
TransfectionoptimizationofHumanMesenchymalStemCells(hMCS)
Plasmidtransfection.
Lipofectamine®LTXinthepresenceofPLUS™Reagentisanexcellentchoicefortransfectionofhumancancercelllinesaswellasprimaryandstemcells.Thereagentenablesthedeliveryofhightransfectionperformancewithoutassociatedcytotoxicity
Lipofectamine®LTXReagent,withPlus™Reagent,efficientlytransfectsprimaryneuralprogenitorcells
Asimple,rapid,serum-compatibleprotocol
Lipofectamine®LTXtransfectionprotocol.
Lipofectamine®LTXReagent,withPLUS™Reagent,efficientlytransfectsprimaryneuralprogenitorcells
Lipofectamine®LTXTransfectionProcedureDetails
Lipofectamine®LTXTransfectionWorkflow
Specifications
| CellType: | EstablishedCellLines, Hard-to-TransfectCells, PrimaryCells, StemCells |
|---|---|
| SampleType(Specific): | PlasmidDNA, RNAiPlasmids(shRNA,miR) |
| Classification: | AnimalOrigin-Free |
| TransfectionTechnique: | Lipid-BasedTransfection |
| SerumCompatible: | Yes |
| HighThroughputCompatibility: | HighThroughput-Compatible |
| ProductLine: | Lipofectamine® |
| ProductSize: | 1mL |
| ShippingCondition: | WetIce |
Contents&storage
EachunitcontainsonevialofLipofectamine®LTXReagent(1mL)andonevialofPlusReagent(0.85ml).Storeat2–8°C.Guaranteedstablefor6monthswhenproperlystored.
