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联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
Description
Lipofectamine®2000TransfectionReagentisaversatiletransfectionreagentthathasbeenshowntoeffectivelytransfectthewidestvarietyofadherentandsUSPensioncelllines.ResearchersuseLipofectamine®2000ReagentforsiRNA-andshRNA-basedgeneknockdownexperiments,aswellasforgeneexpressionstudies.WithLipofectamine®2000TransfectionReagent,you"llget:
•Exceptionaltransfectionefficiencyinthebroadestrangeofcelllinesandthehighestlevelsofrecombinantproteinexpression(seetable)
•Superiorperformanceforco-transfectionofsiRNAandplasmidDNA
•Provenefficacyinthepresenceofserum—eliminatestheneedtochangemediafollowingtransfection
•Reliableperformanceforhigh-throughputapplications
•Thebestchoiceforestablishingstablecelllines
Ahigh-performancetransfectionreagentforgeneexpressionandgenesilencing
Lipofectamine®2000TransfectionReagentworkseffectivelywithallcommoncelllinesaswellaswithmanychallengingones,andcanbeusedinmediawithorwithoutserum.Forgenesilencing,Lipofectamine®2000TransfectionReagent"shigh-efficiencytransfectionsprovidethehighlevelsofgeneknockdownneededtoproduceconvincingresults.Lipofectamine®2000TransfectionReagentisthenumberonechoiceforco-transfection,givenitseffectivenessfortransfectingbothsiRNAandplasmidDNA.Lipofectamine®2000TransfectionReagentiseasytouse—simplymixwithnucleicacidandaddtocellculture.
Idealforhigh-throughputwork
SimplicityandspeedcombinedwithhightransfectionefficiencymakeLipofectamine®2000TransfectionReagentidealfortransientproteinexpressionorhigh-throughputRNAitransfections.Transfectionconditionscanbeeasilyestablishedforautomatedorroboticsystemsusedinsuchapplications.
ForResearchUseOnly.Notforuseindiagnosticprocedures.
Figures
Figure2-Lipofectamineâ„¢2000TransfectionReagentcombinedwithStealthâ„¢RNAiresultinefficientluciferaseknockdown
Lipofectamine®2000transfectionexample
GFPexpression
Figure5-Transfectneuronalorstemcellsandachievegreattransfectionefficiencies
Lipofectamineâ„¢2000celllineperformance
Plasmidtransfection
Figure3-Partiallist*ofcelllinessuccessfullytransfectedwithLipofectamineâ„¢2000TransfectionReagentandobservedtransfectionefficiencies
Lipofectamine®2000transfectionprotocol
Specifications
| CellType: | EstablishedCellLines, Hard-to-TransfectCells, PrimaryCells, StemCells |
|---|---|
| SampleType(Specific): | PlasmidDNA, RNAiPlasmids(shRNA,miR), SyntheticsiRNA |
| TransfectionTechnique: | Lipid-BasedTransfection |
| SerumCompatIBLe: | Yes |
| HighThroughputCompatibility: | HighThroughput-Compatible |
| ProductLine: | Lipofectamine® |
| ProductSize: | 0.75mL |
| ShippingCondition: | ApprovedforshipmentatRoomTemperatureoronWetIce |
| GreenFeatures: | Sustainablepackaging |
Contents&storage
Containsonevial(0.75ml)Lipofectamine®2000Reagent.Storeat2–8°C.Donotfreeze.
