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联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
Description
Dulbecco"sModifiedEagleMedium(DMEM)isawidelyusedbasalmediumforsupportingthegrowthofmanydifferentmammaliancells.CellssuccessfullyculturedinDMEMincludeprimaryfibroblasts,neurons,glialcells,HUVECs,andsmoothmusclecells,aswellascelllinessuchasHeLa,293,Cos-7,andPC-12.WeofferavarietyofGibco®DMEMmodificationsforarangeofcellcultureapplications.Findtherightformulationusingthemediaselectortool.ThisDMEMismodifiedasfollows:
| With | Without |
| •LowGlucose | •SodiumBicarbonate |
| •L-glutamine | •HEPES |
| •SodiumPyruvate | |
| •PhenolRed |
Thecompleteformulationisavailable.
DMEMisuniquefromothermediaasitcontains4timestheconcentrationofaminoacidsandvitaminsthantheoriginalEagle"sMinimalEssentialMedium.DMEMwasoriginallyformulatedwithlowglucose(1g/L)andsodiumpyruvate,butisoftenusedwithhigherglucoselevels,withorwithoutsodiumpyruvate.
ProductIntendedUse
Forinvitrodiagnosticuse.CAUTION:Notforhumanoranimaltherapeuticuse.Usesotherthantheintendedusemaybeaviolationoflocallaw.
cGMPManufacturingandQualitySystem
Gibco®DMEMismanufacturedatacGMPcompliantfacility,locatedinGrandIsland,NewYork.ThefacilityisregisteredwiththeFDAasamedicaldevicemanufacturerandiscertifiedtoISO13485standards.Forsupplychaincontinuity,weofferacomparableGibco®DMEMproductmadeinourScotlandfacility(31600-083).ThisfacilityisregisteredwiththeFDAasamedicaldevicemanufacturerandiscertifiedtotheISO13485standard.
DMEMcontainsnoproteins,lipids,orgrowthfactors.Therefore,DMEMrequiressupplementation,commonlywith10%FetalBovineSerum(FBS).DMEMusesasodiumbicarbonatebuffersystem(3.7g/L)andthereforerequiresa5-10%CO2environmenttomaintainphysiologicalpH.
PowderformsofGibco®cellculturemediumrequiresodiumbicarbonatesupplementation,pHadjustment,andfiltrationatthetimeofpreparation(seeprotocolfordetails).
ForInVitroDiagnosticUse.
Specifications
| Glutamine: | L-Glutamine |
|---|---|
| PhenolRedIndicator: | PhenolRed |
| Form: | Powder |
| Glucose: | LowGlucose |
| SerumSupplementation: | StandardSerumSupplementation |
| HEPESBuffer: | NoHEPES |
| SodiumPyruvateAdditive: | SodiumPyruvate |
| ProductLine: | Gibco® |
| ProductSize: | 10x1L |
| SodiumBicarbonateBuffer: | NoSodiumBicarbonate |
| ShippingCondition: | RoomTemperature |
Contents&storage
Storageconditions:2-8°C
Shippingconditions:Ambient
Shelflife:36monthsfromdateofmanufacture
Shippingconditions:Ambient
Shelflife:36monthsfromdateofmanufacture
Mediaformulations
31600-DMEM,powder,lowglucose,pyruvate
