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联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
Description
DMEM(Dulbecco"sModifiedEagleMedium)isawidelyusedbasalmediumforsupportingthegrowthofmanydifferentmammaliancells.CellssuccessfullyculturedinDMEMincludeprimaryfibroblasts,neurons,glialcells,HUVECs,andsmoothmusclecells,aswellascelllinessuchasHeLa,293,Cos-7,andPC-12.LifeTechnologiesoffersavarietyofDMEMmodificationsforarangeofcellcultureapplications.Findtherightformulationusingthemediaselectortool.ThisDMEMismodifiedasfollows:
| With | Without |
| •HighGlucose | •HEPES |
| •L-glutamine | |
| •PhenolRed | |
| •SodiumPyruvate |
Thecompleteformulationisavailable.
UsingDMEM
DMEMisuniquefromothermediaasitcontains4timestheconcentrationofaminoacidsandvitaminsthantheoriginalEagle"sMinimalEssentialMedium.DMEMwasoriginallyformulatedwithlowglucose(1g/L)andsodiumpyruvate,butisoftenusedwithhigherglucoselevels,withorwithoutsodiumpyruvate.DMEMcontainsnoproteins,lipids,orgrowthfactors.Therefore,DMEMrequiressupplementation,commonlywith10%FetalBovineSerum(FBS).DMEMusesasodiumbicarbonatebuffersystem(3.7g/L),andthereforerequiresa5–10%CO2environmenttomaintainphysiologicalpH.
Productuse
Forhumanexvivotissueandcellcultureprocessingapplications.CAUTION:Whenusedasamedicaldevice,Federallawrestrictsthisdevicetosalebyorontheorderofaphysician.CustomersusingGibco®DMEMinamanufacturingprocess,whohaveasubmissionwiththeFDA,mayrequestaletterofauthorizationfromLifeTechnologiestoreferenceourTypeIIDrugMasterFile(DMF).
cGMPmanufacturingandqualitysystem
DMEMismanufacturedatacGMP-compliantfacilitylocatedinGrandIsland,NewYork.ThefacilityisregisteredwiththeFDAasamedicaldevicemanufacturerandiscertifiedtoISO13485standards.Forsupplychaincontinuity,LifeTechnologiesoffersanidenticalDMEMproductmadeinourScotlandfacility(41966-029).ThisfacilityisregisteredwiththeFDAasamedicaldevicemanufacturerandiscertifiedtotheISO13485standard.
Specifications
| Glutamine: | L-Glutamine |
|---|---|
| PhenolRedIndicator: | PhenolRed |
| Form: | Liquid |
| Glucose: | HighGlucose |
| SerumSupplementation: | StandardSerumSupplementation |
| HEPESBuffer: | NoHEPES |
| SodiumPyruvateAdditive: | SodiumPyruvate |
| ProductLine: | Gibco® |
| ProductSize: | 1,000mL |
| Volume(Metric): | 1000ml |
| GreenFeatures: | Sustainablepackaging |
| ShippingCondition: | RoomTemperature |
Contents&storage
Storageconditions:2-8°C.Protectfromlight
Shippingconditions:Ambient
Shelflife:12monthsfromdateofmanufacture
Shippingconditions:Ambient
Shelflife:12monthsfromdateofmanufacture
Mediaformulations
11995-DMEM,highglucose,pyruvate
