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蚂蚁淘在线 / 品牌中心 / Gibco / Gibco/SILAC Advanced DMEM/F-12 Flex Media, no glucose, no phenol red/500 mL/A2494301
商品描述
Description
SILACAdvancedDMEM/F-12FlexisAdvancedDMEM/F-12withoutL-arginine,L-glutamine,andL-lysineforuseduringSILACproteinlabelingwithstableisotopiclabeledlysineand/orarginine.AdvancedDMEM/F-12isawidelyusedbasalmediumforsupportingthegrowthofmanydifferentmammaliancells,whichreducestheFBSrequirementsby50-90%withoutanychangesingrowthrateormorphology.Whensupplementedwith1-2%FBS,AdvancedDMEM/F-12Flexiscapableofsupportingcellularproliferationandmaximumcelldensitiescomparabletotheconventionalbasalformulationsupplementedwith5-10%FBS.CellssuccessfullyculturedinAdvancedDMEM/F-12withnoadaptationincludeJurkat,WI-38,SP2,Vero,andMRC-5.WeofferavarietyofDMEM/F-12modificationsforarangeofapplications.Findtherightformulationusingthemediaselectortool.ThisAdvancedDMEM/F-12ismanufacturedasfollows:
| With | Without |
| •Non-essentialaminoacids | •Glucose |
| •Sodiumpyruvate | •Phenolred |
| •L-arginine | |
| •L-glutamine | |
| •L-lysine | |
| •HEPES | |
AdvancedDMEM/F-12isuniquefromothermediaduetoadditionofthefollowingingredientstoallowforserumreduction:ethanolamine,glutathione,ascorbicacid,insulin,transferrin,AlbuMAX®Ilipid-richbovineserumalbuminforcellculture,andthetraceelementssodiumselenite,ammoniummetavanadate,cupricsulfate,andmanganouschloride.AdvancedDMEM/F-12typicallyrequiressupplementationwith1-5%FetalBovineSerumand4mML-glutamineorGlutaMAX™supplement(optional).SILACproteinlabelingexperimentsshouldbeconductedusingGibco™DialyzedFBS.TheFBSconcentrationmustbeoptimizedforeachcelllinetoobtainmaximumserumreduction.AdvancedDMEM/F-12usesasodiumbicarbonatebuffersystem(3.7g/L),andthereforerequiresa5-10%CO2environmenttomaintainphysiologicalpH.
cGMPManufacturingandQualitySystem
SILACAdvancedDMEM/F-12FlexismanufacturedatacGMP-compliantfacilitylocatedinGrandIsland,NewYork.ThefacilityisregisteredwiththeFDAasamedicaldevicemanufacturerandiscertifiedtoISO13485standards.
ForResearchUseorFurtherManufacturing.Notforuseindiagnosticprocedures.
Specifications
| Glutamine: | NoGlutamine |
|---|---|
| PhenolRedIndicator: | NoPhenolRed |
| Form: | Liquid |
| HEPESBuffer: | NoHEPES |
| SodiumPyruvateAdditive: | SodiumPyruvate |
| SerumSupplementation: | Advanced(ReducedSerum) |
| Glucose: | NoGlucose |
| ProductLine: | Gibco® |
| ProductSize: | 500mL |
| Volume(Metric): | 500.0mL |
| GreenFeatures: | Sustainablepackaging |
Contents&storage
Storeinrefrigerator(2–8°C)andprotectfromlight.
Shelflife:12monthsfromdateofmanufacture.
Shelflife:12monthsfromdateofmanufacture.
