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联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
Description
DMEM/F-12(Dulbecco"sModifiedEagleMedium/NutrientMixtureF-12)isawidelyusedbasalmediumforsupportingthegrowthofmanydifferentmammaliancells.CellssuccessfullyculturedinDMEM/F-12includeMDCK,glialcells,fibroblasts,humanendothelialcells,andratfibroblasts.WeofferavarietyofDMEM/F-12modificationsforarangeofcellcultureapplications.Findtherightformulationusingthemediaselectortool.ThisDMEM/F-12ismodifiedasfollows:
| With | Without |
| •L-glutamine | •SodiumBicarbonate |
| •HEPES | |
| •PhenolRed |
Thecompleteformulationisavailable.
UsingDMEM/F-12
DMEM/F-12isa1:1mixtureofDMEMandHam"sF-12.ThisformulationcombinesDMEM"shighconcentrationsofglucose,aminoacids,andvitaminswithF-12"swidevarietyofcomponents.DMEM/F-12containsnoproteins,lipids,orgrowthfactors.Therefore,DMEM/F-12mayrequiresupplementation,commonlywith10%FetalBovineSerum(FBS).DMEM/F-12usesasodiumbicarbonatebuffersystemandthereforerequiresa5–10%CO2environmenttomaintainphysiologicalpH.PowderformsofGibco®cellculturemediarequiresodiumbicarbonatesupplementation,pHadjustment,andfiltrationatthetimeofpreparation(seeprotocolfordetails).
cGMPmanufacturingandqualitysystem
DMEM/F-12ismanufacturedatacGMP-compliantfacilitylocatedinGrandIsland,NewYork.ThefacilityisregisteredwiththeFDAasamedicaldevicemanufacturerandiscertifiedtoISO13485standards.Forsupplychaincontinuity,weofferacomparableDMEM/F-12productmadeinourScotlandfacility(42400-010).ThisfacilityisregisteredwiththeFDAasamedicaldevicemanufacturerandiscertifiedtotheISO13485standard.
ForInVitroDiagnosticUse.
Specifications
| Glutamine: | L-Glutamine |
|---|---|
| PhenolRedIndicator: | PhenolRed |
| Form: | Powder |
| HEPESBuffer: | HEPES |
| SodiumPyruvateAdditive: | SodiumPyruvate |
| SerumSupplementation: | StandardSerumSupplementation |
| Glucose: | HighGlucose |
| ProductLine: | Gibco® |
| ProductSize: | 10x1L |
| ShippingCondition: | RoomTemperature |
Contents&storage
Storageconditions:2-8°C
Shippingconditions:Ambient
Shelflife:36monthsfromdateofmanufacture
Shippingconditions:Ambient
Shelflife:36monthsfromdateofmanufacture
Mediaformulations
12400-DMEM/F-12,powder,HEPES
