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联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
Description
AdvancedDMEM/F-12(Dulbecco"sModifiedEagleMedium/Ham"sF-12)isawidelyusedbasalmediumthatallowsthecultureofmammaliancellswithreducedFetalBovineSerum(FBS)supplementation.ComparedtoclassicDMEM/F-12,serumsupplementationcanbereducedby50–90%withnochangeingrowthrateormorphology.CellssuccessfullyculturedinAdvancedDMEM/F-12,withnoadaptation,includeMRC-5,SP2,Vero,WI-38andJurkat.ThisAdvancedDMEM/F-12ismanufacturedasfollows:
| With | Without |
| •Glucose | •L-glutamine |
| •NonEssentialAminoAcids | •HEPES |
| •Sodiumpyruvate | |
| •PhenolRed |
Thecompleteformulationisavailable.
UsingAdvancedDMEM/F-12
AdvancedDMEM/F-12isuniquefromothermediaduetoadditionofthefollowingingredientstoallowforserumreduction:ethanolamine,glutathione,ascorbicacid,insulin,transferrin,AlbuMAX®IIlipid-richbovineserumalbuminforcellculture,andthetraceelementssodiumselenite,ammoniummetavanadate,cupricsulfate,andmanganouschloride.AdvancedDMEM/F-12requiressupplementationwith1–5%FetalBovineSerumand4mML-glutamineorGlutaMAX™supplement.Manycelllinesdonotrequireadaptationtothismedia.TheFBSconcentrationmustbeoptimizedforeachcelllinetoobtainmaximumserumreduction.AdvancedAdvancedDMEM/F-12usesasodiumbicarbonatebuffersystem(3.7g/L)andthereforerequiresa5–10%CO2environmenttomaintainphysiologicalpH.
cGMPmanufacturingandqualitysystem
Forsupplychaincontinuity,wemanufactureAdvancedDMEM/F-12attwoseparatefacilitieslocatedinGrandIsland,NYandScotland,UK.BothsitesarecompliantwithcGMPmanufacturingrequirements,arecertifiedtoISO13485,andareregisteredwiththeFDAasmedicaldevicemanufacturers.
ForResearchUseOnly.Notforuseindiagnosticprocedures.
Specifications
| Glutamine: | NoGlutamine |
|---|---|
| PhenolRedIndicator: | PhenolRed |
| Form: | Liquid |
| HEPESBuffer: | NoHEPES |
| SodiumPyruvateAdditive: | SodiumPyruvate |
| SerumSupplementation: | Advanced(ReducedSerum) |
| Glucose: | HighGlucose |
| ProductLine: | Gibco® |
| ProductSize: | 500mL |
| Volume(Metric): | 500ml |
| GreenFeatures: | Sustainablepackaging |
| ShippingCondition: | RoomTemperature |
Contents&storage
Storageconditions:2-8°C.Protectfromlight
Shippingconditions:Ambient
Shelflife:12monthsfromdateofmanufacture
Shippingconditions:Ambient
Shelflife:12monthsfromdateofmanufacture
Mediaformulations
12634-AdvancedD-MEM/F-12
