Platecharacteristics:

Transparent

Contents:

• Coatingantibodies
• Biotinylateddetectorantibodies
• Phi-labeledgoatanti-biotinantibodies
• Activatorsolutions
• Blockingstocksolution
• DilutionbufferT
• Tween-20
• TransparentELISPOTplateswithlidsandcoverslips
• Detailedmanual

Referenceslist:

• AutoreactiveTcellresponsesshowproinflammatorypolarizationindiabetesbutaregulatoryphenotypeinhealth.Arif,Sefina,etal.J.Clin.Invest.2004Feb;113:451-63
• Self-glycolipidsmodulatedendriticcellschangingthecytokineprofilesofcommittedautoreactiveTcells.Buschard,Karsten,etal.PLoSONE2012;7:e52639
• Developmentoftype1diabetesdespiteseverehereditaryB-lymphocytedeficiency.Martin,S,etal.N.Engl.J.Med.2001Oct4;345:1036-40
• DevelopmentofnovelLL-37derivedantimicrobialpeptideswithLPSandLTAneutralizingandantimicrobialactivitiesfortherapeuticapplication.Nell,MarjaJ,etal.Peptides2006Apr;27:649-60
• AssociationofT-cellreactivitywithbeta-cellfunctioninrecentonsettype1diabetespatients.Pfleger,Christian,etal.J.Autoimmun.2010Mar;34:127-35
• Beta-cellfunctioninnew-onsettype1diabetesandimmunomodulationwithaheat-shockproteinpeptide(DiaPep277):arandomised,double-blind,phaseIItrial.Raz,I,etal.Lancet2001Nov24;358:1749-53
• IsletantigenspecificIL-10+immuneresponsesbutnotCD4+CD25+FoxP3+cellsatdiagnosispredictglycemiccontrolintype1diabetes.Sanda,Srinath,etal.Clin.Immunol.2008May;127:138-43
• CD8T-cellreactivitytoisletantigensisuniquetotype1whileCD4T-cellreactivityexistsinbothtype1andtype2diabetes.Sarikonda,Ghanashyam,etal.J.Autoimmun.2014May;50:77-82
• ComparisonofcytokineELISpotassayformatsforthedetectionofisletantigenautoreactiveTcells.ReportofthethirdimmunologyofdiabetessocietyT-cellworkshop.Schloot,NanetteC,etal.J.Autoimmun.2003Dec;21:365-76
• AutoantibodiesandassociatedT-cellresponsestodeterminantswithinthe831-860regionoftheautoantigenIA-2inType1diabetes.Weenink,SM,etal.J.Autoimmun.2009Sep;33:147-54
• Humanmesenchymalstemcellsmodulatecellularimmuneresponsetoisletantigenglutamicaciddecarboxylaseintype1diabetes.Zanone,MariaM,etal.J.Clin.Endocrinol.Metab.2010Aug;95:3788-97
• RedirectionofhumanautoreactiveT-cellsUponinteractionwithdendriticcellsmodulatedbyTX527,ananalogof1,25dihydroxyvitaminD(3).vanHalteren,AstridGS,etal.Diabetes2002Jul;51:2119-25

U-CyTech为干扰素提供基于CPE的抗病毒测定（AVA）测试，也称为细胞病变效应（CPE）抑制测定。在该体外生物测定中，将一个单位定义为将病毒感染的CPE降低50％的干扰素的量。将测试样品的抗病毒生物活性与国际或实验室标准制剂进行比较，并以国际单位（IU）或实验室单位（LU）/ ml表示。 人，大鼠和小鼠干扰素的生物活性分别通过人肺上皮细胞（HEp-2），大鼠胚胎细胞（RATEC）和小鼠成纤维细胞（L929）受到水泡性口腔炎病毒（VSV）攻击来测量。