Description:

IL-4specificspotsproducedby2x10E5mouse(BALB/c)splenocytes.
Stimulation:PMA/ionomycin.

Platecharacteristics:

PVDF

Contents:

• Coatingantibodies
• Biotinylateddetectorantibodies
• Streptavidin-HorserADIshPeroxidase
• AECcoloringsystem
• BlockingstocksolutionR
• DilutionbufferR
• Tween-20
• Detailedmanual

Referenceslist:

• VaccinationofmicewithrecombinantbaculovirusexpressingspikeornucleocapsidproteinofSARS-likecoronavirusgenerateshumoralandcellularimmuneresponses.Bai,Bingke,etal.Mol.Immunol.2008Feb;45:868-75
• Protectiveeffectofenterovirus‑71(EV71)virus‑likeparticlevaccineagainstlethalEV71infectioninaneonatalmousemodel.Cao,Lei,etal.MolMedRep2015Aug;12:2473-80
• Liposome-basedpolymercomplexasanoveladjuvant:enhancementofspecificantibodyproductionandisotypeswitch.Chen,Chia-Hung,etal.IntJNanomedicine2012;7:607-21
• RemovingN-terminalsequencesinpre-S1domainenhancedantibodyandB-cellresponsesbyanHBVlargesurfaceantigenDNAvaccine.Ge,Guohong,etal.PLoSONE2012;7:e41573
• InductionofspecificimmuneresponsesbysevereacuterespiratorysyndromecoronavirusspikeDNAvaccinewithorwithoutinterleukin-2immunizationusingdifferentvaccinationroutesinmice.Hu,Hui,etal.Clin.VaccineImmunol.2007Jul;14:894-901
• Characterizationoftheimmuneresponseselicitedbybaculovirus-basedvectorvaccinesagainstinfluenzavirushemagglutinin.Hu,Zhi-peng,etal.ActaPharmacol.Sin.2012Jun;33:783-90
• AsingleimmunizationwithHADNAvaccinebyelectroporationinducesearlyprotectionagainstH5N1avianinfluenzaviruschallengeinmice.Zheng,Liyun,etal.BMCInfect.Dis.2009;9:17

U-CyTech为干扰素提供基于CPE的抗病毒测定（AVA）测试，也称为细胞病变效应（CPE）抑制测定。在该体外生物测定中，将一个单位定义为将病毒感染的CPE降低50％的干扰素的量。将测试样品的抗病毒生物活性与国际或实验室标准制剂进行比较，并以国际单位（IU）或实验室单位（LU）/ ml表示。 人，大鼠和小鼠干扰素的生物活性分别通过人肺上皮细胞（HEp-2），大鼠胚胎细胞（RATEC）和小鼠成纤维细胞（L929）受到水泡性口腔炎病毒（VSV）攻击来测量。