Description:

AntibodiestotheSSAantigen(alsoknownasRoantigen)areoneofthemostfrequentSEROlogicalMarkersofautoimmunityinrheumaticdiseases.Theyappearin60-70%ofpatientswithSjögren’ssyndrome(SS),30-40%ofpatientswithsystemiclupuserythematosus(SLE),and3-5%ofpatientswithrheumatoidarthritis(RA).TheSSAantigeniscomprisedofanacidic60kDaproteinthatmayexistincomplexwithRNA(80-112bases).However,RNAisnotrequiredforSSAantigenicity.TheSSAantigenispredominantlycytoplasmic,andnotlocatedinthenucleus.Therefore,patientswithantibodytoSSAmaybeANAnegativeonroutinetesting.Approximately62%ofANAnegativelupuspatientshaveantibodiestoSSA.Twotypesofanti-Ro/SSAantibodieshavebeenidentified;Ro/SSAantigensof60kDaand52kDa.Anti-SSA-60kDaantibodiesarelinkedtocertaindisorderssuchasSS,SLE,neonatallupusandcongenitalheartblock.Clinically,thepresenceofaSSA52hasbeenreportedinawidevarietyofdiseases,includesinflammatorymyositis,primarybiliarycirrhosisandSS.

Principle:

AutoimmuneELISAkitsmeasureautoimmuneantibodiesintheserum.Itisbasedontheprincipleofasolidphaseenzyme-linkedimmunosorbentassay.Theassayutilizesaspecificantigenforimmobilizationonthemicrotiterwellsandanti-humanIgGantibodyconjugatedtohorserADIshperoxidase(HRP)fordetection.Thetestsampleisallowedtoreactsimultaneouslywiththetwocomponents,resultingin autoimmuneantibodiesbeingsandwichedbetweenthesolidphaseandenzyme-linkedantibodies.Afterincubation,thewellsarewashedtoremoveunbound-labeledantibodies.AHRPsubstrate,TMB,isaddedtoresultinthedevelopmentofabluecolor.ThecolordevelopmentisthenstoppedwiththeadditionofStopSolutionchangingthecolortoyellow.Theconcentrationofautoimmuneantibodies isdirectlyproportionaltothecolorintensityofthetestsample.Absorbanceismeasuredspectrophotometricallyat450nm.

Data:

Signosis基因表达  是利用来自基因的信息指导功能性基因产物合成的过程。这些产品通常是蛋白质。基因表达分析对于理解基因及其蛋白产物的功能，以及对影响基本生物学过程的复杂调控网络的清晰了解至关重要。Signosis  提供了许多用于基因表达研究的测定产品，包括用于监控由TF上调或下调的基因表达的cDNA板阵列，以及用于分析基因表达的单细胞PCR试剂盒，可直接分析细胞无需RNA分离即可进行裂解。 SARS-CoV-2实时PCR检测试剂盒单细胞基因表达分析印迹分析RNA的cDNA板阵列细胞裂解液的cDNA板阵列