Description:

Rapidlygrowingtumorsresultinhypoxicregions.Adaptiveresponsesofmostcellstohypoxiaare(1)toproduceVEGFandotherhypoxia-inducedangiogeniccytokinesthatpromoteincreasedtissuevascularization,therebyincreasingtissueoxygenation,and(2)toswitchmetabolicallyfromoxidativephosphorylationtoanaerobicglycolysis.Hypoxia-inducIBLefactor1(HIF-1)isanoxygen-regulatedtranscriptionalactivatorthatplaysessentialrolesintheprocess.TheHIF-1alphasubunitisoxygen-dependentubiquitinationandproteasomaldegradation.Inaddition,cytokinesincludinginterleukin-1(IL-1)andtumornecrosisfactor-alpha(TNF-alpha)stimulateHIF-1dependentgeneexpression.HIF-1increasestheexpressionofseveralgenesthatpromotebloodflowandinflammation,suchasvascularendothelialgrowthfactor(VEGF). TogetabetteroverviewofgeneexpressionofHIF-1 regulatedgenes,SignosishasdevelopedaCDNAPlateArraywhichcouplesitsCL^TM cDNAsynthesiskitwhichallowsreversetranscriptiondirectlyfromcelllysateswithoutRNApreparation.Thisplate-basedassaysallowsprofilingtheexpressionof20+HIF-1-regulatedgenesdirectlyincelllysates.Thisassayprovidesquantitativedata,allowingresearcherstoscreenmultiplesamples,aswellashighsensitivity,allowingresearcherstouselesssample. 

ApplicableGrid:

ListofApplicableGenes

BCL2	HIF-2a	PDGF-A	BCL2	HIF-2a	PDGF-A	BCL2	HIF-2a	PDGF-A	BCL2	HIF-2a	PDGF-A
Beta-actin	HPRT	PDK1	Beta-actin	HPRT	PDK1	Beta-actin	HPRT	PDK1	Beta-actin	HPRT	PDK1
CA9	IGF-II	NDRG1	CA9	IGF-II	NDRG1	CA9	IGF-II	NDRG1	CA9	IGF-II	NDRG1
CTSD	Leptin	SAG	CTSD	Leptin	SAG	CTSD	Leptin	SAG	CTSD	Leptin	SAG
BHLHB2	MMP1	SLC6A8	BHLHB2	MMP1	SLC6A8	BHLHB2	MMP1	SLC6A8	BHLHB2	MMP1	SLC6A8
Glut-1	MXI-1	VEGF	Glut-1	MXI-1	VEGF	Glut-1	MXI-1	VEGF	Glut-1	MXI-1	VEGF
Glut-3	NDRG2	VHL	Glut-3	NDRG2	VHL	Glut-3	NDRG2	VHL	Glut-3	NDRG2	VHL
HIF-1a	PAI-1	Blank	HIF-1a	PAI-1	Blank	HIF-1a	PAI-1	Blank	HIF-1a	PAI-1	Blank

Signosis基因表达  是利用来自基因的信息指导功能性基因产物合成的过程。这些产品通常是蛋白质。基因表达分析对于理解基因及其蛋白产物的功能，以及对影响基本生物学过程的复杂调控网络的清晰了解至关重要。Signosis  提供了许多用于基因表达研究的测定产品，包括用于监控由TF上调或下调的基因表达的cDNA板阵列，以及用于分析基因表达的单细胞PCR试剂盒，可直接分析细胞无需RNA分离即可进行裂解。 SARS-CoV-2实时PCR检测试剂盒单细胞基因表达分析印迹分析RNA的cDNA板阵列细胞裂解液的cDNA板阵列