Description:

NFkBisaubiquitoustranscriptionfactorthatplaysakeyroleincellularresponsestostimulisuchasstress,cytokines,freerADIcals,ultravioletirradiation,oxidizedLDL,andbacterialorviralantigens.IncorrectregulationofNFkBhasbeenlinkedtocancer,inflammatoryandautoimmunediseases,septicshock,viralinfection,andimproperimmunedevelopment.WhenNFkBisactivated,itisdissociatedfromitsinhibitorIkBandmovesfromthecytoplasmtothenucleus,whereitbindstotargetDNAelementsandpositivelyregulatesthetranscriptionofgenesinvolvedinimmuneandinflammatoryresponses,cellgrowthcontrol,andapoptosis.TogetabetteroverviewofgeneexpressionofNFkBregulatedgenes,SignosishasdevelopedaCDNAPlateArraywhichcouplesitsCL^TM cDNAsynthesiskitwhichallowsreversetranscriptiondirectlyfromcelllysateswithoutRNApreparation.Thisplate-basedassaysallowsprofilingtheexpressionof20+NFkB-regulatedgenesdirectlyincelllysates.Thisassayprovidesquantitativedata,allowingresearcherstoscreenmultiplesamples,aswellashighsensitivity,allowingresearcherstouselesssample. 

Principle:

UnlikeconventionalreversetranscriptionwhichneedstotalRNA,SignosishasdevelopedaCL(TM)cDNAsynthesis-basedplatearray.ItcansynthesizecDNAprobesthroughreversetranscriptiondirectlyincelllysateswithoutRNApreparation.ThesynthesizedcDNAprobesarethenappliedforaplatearrayhybridization.Targetedgenesarespecificallycapturedontoindividualwellsonaplatethroughapre-coatedgene-specificoligonucleotide.ThecapturedcDNAsarefurtherdetectedwithstreptavidin-HRP.Luminescenceisreportedasrelativelightunits(RLUs)onamicroplateluminometer.Theexpressionlevelofgenesisdirectlyproportionaltotheluminescentintensity.

 

 

Data:

HumanNFkB-regulatedcDNAplatearrayanalysis. HeLacellsweretreatedwith(redbars)orwithout(lightblue)10ng/mlTNFfor30min. Cellswerecollectedforprofiling23geneswiththecDNAplatearrayassay(top).TimecourseofIL-6inductiontoTNFatreatmentinHeLacellswasmonitoredwiththecDNAplatearrayassay.

Signosis基因表达  是利用来自基因的信息指导功能性基因产物合成的过程。这些产品通常是蛋白质。基因表达分析对于理解基因及其蛋白产物的功能，以及对影响基本生物学过程的复杂调控网络的清晰了解至关重要。Signosis  提供了许多用于基因表达研究的测定产品，包括用于监控由TF上调或下调的基因表达的cDNA板阵列，以及用于分析基因表达的单细胞PCR试剂盒，可直接分析细胞无需RNA分离即可进行裂解。 SARS-CoV-2实时PCR检测试剂盒单细胞基因表达分析印迹分析RNA的cDNA板阵列细胞裂解液的cDNA板阵列