Description:

Rapidlygrowingtumorsresultinhypoxicregions.Adaptiveresponsesofmostcellstohypoxiaare(1)toproduceVEGFandotherhypoxia-inducedangiogeniccytokinesthatpromoteincreasedtissuevascularization,therebyincreasingtissueoxygenation,and(2)toswitchmetabolicallyfromoxidativephosphorylationtoanaerobicglycolysis.Hypoxia-inducIBLefactor1(HIF-1)isanoxygen-regulatedtranscriptionalactivatorthatplaysessentialrolesintheprocess.TheHIF-1alphasubunitisoxygen-dependentubiquitinationandproteasomaldegradation.Inaddition,cytokinesincludinginterleukin-1(IL-1)andtumornecrosisfactor-alpha(TNF-alpha)stimulateHIF-1dependentgeneexpression.HIF-1increasestheexpressionofseveralgenesthatpromotebloodflowandinflammation,suchasvascularendothelialgrowthfactor(VEGF). 

ApplicableGrid:

ListofApplicableGenes

1

2

3

4

5

6

7

8

9

10

11

12

A

ANGPTL4

Glut-3

PDGF-A

ANGPTL4

Glut-3

PDGF-A

ANGPTL4

Glut-3

PDGF-A

ANGPTL4

Glut-3

PDGF-A

B

18SrRNA

HIF-1a

PDK1

18SrRNA

HIF-1a

PDK1

18SrRNA

HIF-1a

PDK1

18SrRNA

HIF-1a

PDK1

C

BCL2

HIF-2a

RTP

BCL2

HIF-2a

RTP

BCL2

HIF-2a

RTP

BCL2

HIF-2a

RTP

D

CREBP

HPRT

SAG

CREBP

HPRT

SAG

CREBP

HPRT

SAG

CREBP

HPRT

SAG

E

CTSD

IGF-II

UCP3

CTSD

IGF-II

UCP3

CTSD

IGF-II

UCP3

CTSD

IGF-II

UCP3

F

EP300

Leptin

VEGF

EP300

Leptin

VEGF

EP300

Leptin

VEGF

EP300

Leptin

VEGF

G

EPO

MMP2

VHL

EPO

MMP2

VHL

EPO

MMP2

VHL

EPO

MMP2

VHL

H

Glut-1

PAI-1

Blank

Glut-1

PAI-1

Blank

Glut-1

PAI-1

Blank

Glut-1

PAI-1

Blank

Signosis基因表达  是利用来自基因的信息指导功能性基因产物合成的过程。这些产品通常是蛋白质。基因表达分析对于理解基因及其蛋白产物的功能，以及对影响基本生物学过程的复杂调控网络的清晰了解至关重要。Signosis  提供了许多用于基因表达研究的测定产品，包括用于监控由TF上调或下调的基因表达的cDNA板阵列，以及用于分析基因表达的单细胞PCR试剂盒，可直接分析细胞无需RNA分离即可进行裂解。 SARS-CoV-2实时PCR检测试剂盒单细胞基因表达分析印迹分析RNA的cDNA板阵列细胞裂解液的cDNA板阵列