Description:
CytokinesareessentialmoleculesthatplaycrucialrolesinmanyBIOLOGicalfunctions,includingviralinfection,inflammation,immunity,andhematopoiesis.Cytokinesareproducedbyavarietyofcelltypesinresponsetodifferentstimuli.Inaddition,theexpressionofcytokinegenesappearstoberegulatedbycomplexmechanism.Expressionofonecytokinegenecouldberegulatedbyothercytokines.Dysregulationofcytokinegeneexpressionmaybecausedbychromosomalalterationsorbyinfectionofvirusesthatinduceactivationorinactivationoftheexpressionmachinery.Therefore,expressionprofilingofcytokinegenescouldprovideausefulapproachtouncoverthemechanismunderlyingtheirregulation.ApplicableGrid:
ListofApplicableGenes
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
| A | 18SrRNA | IL-13 | IL-4 | 18SrRNA | IL-13 | IL-4 | 18SrRNA | IL-13 | IL-4 | 18SrRNA | IL-13 | IL-4 |
| B | Eotaxin | IL-14 | IL-5 | Eotaxin | IL-14 | IL-5 | Eotaxin | IL-14 | IL-5 | Eotaxin | IL-14 | IL-5 |
| C | GAPDH | IL-15 | IL6 | GAPDH | IL-15 | IL6 | GAPDH | IL-15 | IL6 | GAPDH | IL-15 | IL6 |
| D | IFNb | IL-16 | IL-7 | IFNb | IL-16 | IL-7 | IFNb | IL-16 | IL-7 | IFNb | IL-16 | IL-7 |
| E | IFNg | IL-17 | TGFa | IFNg | IL-17 | TGFa | IFNg | IL-17 | TGFa | IFNg | IL-17 | TGFa |
| F | IL-10 | IL1A | TGFb | IL-10 | IL1A | TGFb | IL-10 | IL1A | TGFb | IL-10 | IL1A | TGFb |
| G | IL11 | IL2 | TNFa | IL11 | IL2 | TNFa | IL11 | IL2 | TNFa | IL11 | IL2 | TNFa |
| H | IL-12 | IL-3 | VEGFC | IL-12 | IL-3 | VEGFC | IL-12 | IL-3 | VEGFC | IL-12 | IL-3 | VEGFC |
Principle:
Signosis’proprietaryCDNAplatearrayisaplate-basedhybridizationprofilinganalysisformonitoringtheexpressionofdozensofgenesthroughreversetranscriptionofmRNAintocDNA.Likearrayanalyses,totalRNAisfirstreversetranscribedintocDNAinthepresenceofbiotin-dUTPintheassay.Targetedgenesarethenspecificallycapturedontoindividualwellsonaplate,insteadofmembranes,throughapre-coatedgene-specificoligonucleotide.ThecapturedcDNAsarefurtherdetectedwithstreptavidin-HRP.Luminescenceisreportedasrelativelightunits(RLUs)onamicroplateluminometer.Theexpressionlevelofgenesisdirectlyproportionaltotheluminescentintensity.
Data:
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