Description:

IL-1a and IL-1ß are prominent agonists mediating inflammatory and immunomodulatory effects.  Both are produced by macrophages, monocytes and dendritic cells. They are important part of the inflammatory response against infection. They increase the expression of adhesion factors on endothelial cells to enable transmigration of leukocytes to sites of infection.  IL-1ß production in peripheral tissue has also been associated with hyperalgesia (increased sensitivity to pain) associated with fever.

Principle:

Mouse Cytokine ELISA is based on the principle of a solid phase enzyme-linked immunosorbent assay. The assay utilizes rabbit anti-mouse antibodies for immobilization on the microtiter wells and rabbit anti-mouse antibodies along with streptavidin conjugated to horseradish peroxidase (HRP) for detection. The test sample is allowed to react simultaneously with the two antibodies, resulting in the molecules being sandwiched between the solid phase and enzyme-linked antibodies. After incubation, the wells are washed to remove unbound-labeled antibodies. A HRP substrate, TMB, is added to result in the development of a blue color. The color development is then stopped with the addition of Stop Solution changing the color to yellow. The   concentration of VEGF is directly proportional to the color intensity of the test sample. Absorbance is measured spectrophotometrically at 450 nm. 
 

 

 

Data:

 

 

 

Signosis基因表达  是利用来自基因的信息指导功能性基因产物合成的过程。这些产品通常是蛋白质。基因表达分析对于理解基因及其蛋白产物的功能，以及对影响基本生物学过程的复杂调控网络的清晰了解至关重要。Signosis  提供了许多用于基因表达研究的测定产品，包括用于监控由TF上调或下调的基因表达的cDNA板阵列，以及用于分析基因表达的单细胞PCR试剂盒，可直接分析细胞无需RNA分离即可进行裂解。 SARS-CoV-2实时PCR检测试剂盒单细胞基因表达分析印迹分析RNA的cDNA板阵列细胞裂解液的cDNA板阵列