Description:

Angiogenesisprovidesthecruciallinkbetweentheavascularandvascularstatesandisakeyeventforsustainedtumorgrowthandcancerprogression.AngiogenesisasaBIOLOGicalswitchprocessisgovernedbynumerouspro-andanti-angiogenicfactors,includingvascularendothelialgrowthfactor(VEGF),basicfibroblastgrowthfactor(FGFb),epidermalgrowthfactor(EGF),andtransforminggrowthfactor-beta(TGF-b).Themechanismofactionofeachofthesefactorsisdifferent,asaretheiroriginandthestimulifortheirproduction.Theangiogenicswitchreferstothebalancebetweenpro-andanti-angiogenicfactors.Therefore,profilingofthesefactorsiscriticaltounderstandingangiogenesis.Signosis’AngiogenesisELISAStripIProfilingAssayallowssimultaneouslyprofiling8angiogenesiscytokines. Eachwellofthestripiscoatedwithaprimaryantibodyagainstaspecificangiogenesisproteinandtotal8wellsofastriptarget8differentproteins.Thedifferenceoftheseproteinsbetweentwosamplescanbedeterminedthroughdatacomparison.

Principle:

Eachwellofthestripiscoatedwithaspecificcaptureantibodytodetectitscorrespondingcytokineinthesample.Therefore,8differentproteinscanbemeasuressimultaneously.Thetestsamplereactssimultaneouslywithtwoantibodies,resultinginthecytokinesbeingsandwichedbetweenthesolidphaseandenzyme-linkedantibodies.Afterincubation,thewellsarewashedtoremoveunbound-labeledantibodies.TheHRPsubstrate,TMB,isthenaddedandcausesabluecolorchange.ThereactionisthenterminatedwithStopSolution,resultinginayellowcolor.Theconcentrationsofoxidativestresscytokinesaredirectlyproportionaltothecolorintensityofthetestsample.Absorbanceismeasuredspectrophotometricallyat450nm.Theexpressionlevelsofthesecytokinescanbequantitativelycomparedbetweensamples.

 

 

 

Data:

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

HumanAngiogenesisELISAstripIforprofiling8cytokines.HeLacellsweretreatedwith(redbars)orwithout(lightblue)10ng/mlTNF.Conditionedmediawerecollected(top)andcelllysateswereprepared(bottom)forELISAstripprofiling.

 

 

Signosis基因表达  是利用来自基因的信息指导功能性基因产物合成的过程。这些产品通常是蛋白质。基因表达分析对于理解基因及其蛋白产物的功能，以及对影响基本生物学过程的复杂调控网络的清晰了解至关重要。Signosis  提供了许多用于基因表达研究的测定产品，包括用于监控由TF上调或下调的基因表达的cDNA板阵列，以及用于分析基因表达的单细胞PCR试剂盒，可直接分析细胞无需RNA分离即可进行裂解。 SARS-CoV-2实时PCR检测试剂盒单细胞基因表达分析印迹分析RNA的cDNA板阵列细胞裂解液的cDNA板阵列