Description:
WhenoneTFisactivated,itbindstoabiotin-labeledprobe,whichisdesignedbasedontheconsensusbindingsequenceoftheTF.Whentheformedcomplexissubjectedtoelectrophoresis,itrunsslowerthanthefreeprobe,andcanbeeasilydistinguished.Twoormoresamplescanbecomparedanddifferencescanbedeterminedbythedensityoftheshiftedband.Principle:
WhenoneTFisactivated,itbindstoabiotin-labeledprobe,whichisdesignedbasedontheconsensusbindingsequenceoftheTF.Whentheformedcomplexissubjectedtoelectrophoresis,itrunsslowerthanthefreeprobe,andcanbeeasilydistinguished.Twoormoresamplescanbecomparedanddifferencescanbedeterminedbythedensityoftheshiftedband.